Using pharmacological inhibitors that perturb individual aspects of actomyosin dynamics without disassembling the network, we demonstrate that F-actin polymerization is the primary driver of actin retrograde flow, whereas myosin IIA promotes long-term integrity of the IS. Disruption of F-actin retrograde...
因为细胞皮层在不断翻转,报告分子与F-actin的快速可逆结合确保了测量局部F-actin密度快速变化的可行性。 Fig. 1.Development of a fluorescent reporter that measures the local density of membrane-proximal F-actin. 在处于迁移状态的HT-HUVEC中,...
Recently we demonstrated the existence of a phosphatidylinositol 3-kinase (PI3K)–independent F-actin polymerization during neutrophil pseudopod extension. Here we examine the use of the PI3K-dependent and PI3K-independent pathways of activation by the N-formyl peptide receptor and the chemokine receptor...
细胞肌动蛋白以单体和聚合体形式存在于真核细胞内,其中单体小球又称为G-肌动蛋白(Globular Actin,G-actin);多个G-肌动蛋白以双螺旋结构聚合形成纤维化肌动蛋白,称为F-肌动蛋白(Fibros Actin,F-actin),又称为微丝,是细胞骨架最主要的结构组分之一。 G-Actin/F-Actin这2种形式在一定生理条件下相互转换,在...
(1.广东省农业科学院动物卫生研究所，广东省兽医公共卫生公共实验室，广东省畜禽疫病防治研究重点实验室，广州510640；2.华南农业大学兽医学院，广州510642)摘 要：病原入侵宿主细胞，由宿主细胞肌动蛋白(F -a c t i n )聚集引起的细胞骨架重排起着极其重要的作用，本文主要就宿主细胞整合素介导的宿主细胞F -a c...
Since alteration in F-actin level influences on the formation of vacuoles/vesicles during exocytosis/endocytosis in astrocytes, we investigated whether F-actin polymerization involves clasmatodendrosis in the rat hippocampus following status epilepticus (SE). In the present study, vacuoles in clasmato...
Tubulin polymerization assay using >99% pure tubulin, fluorescence basedBK011P96 assays荧光法 二、Cytoskeleton活细胞微丝蛋白F-actin的染色&肌动蛋白聚合分析 #F-actin活细胞染色: 关于actin染色,我们都知道鬼笔环肽衍生物系列产品,可以用于固定后细胞的染色,但是,有没有一种可以染活细胞F-actin的染料呢?研究者对...
Using both genetic and pharmacological approaches, we show that excess F-actin polymerization leads to impaired autophagic activity and the accumulation of dysfunctional mitochondria in the aged brain. Remarkably, we show that treating aged animals with cytoskeletal drugs, to disrupt actin polymerization,...
Phalloidin鬼笔环肽;F-actin肌动蛋白(聚合);G-actin球形肌动蛋白(单体);Actin Polymerization肌动蛋白聚合;Cytochalasin细胞松弛素;CAS NO.:17466-45-4; 产品信息 产品名称 产品编号 规格 价格(元) TRITC Phalloidin TRITC标记鬼笔环肽 MX4405-300T 300T ...
The toxin triphenyl tin (TPT), Sn(CH)caused a rapid decrease in the F-actin content of promyelocytic human leukemia cells (HL-60) chemically differentiated to neutrophils. Prior incubation (2 min) of the cells with 10 M TPT did not modify the extent of actin polymerization inducible either ...