single immune cell data sets were used to identify marker gene sets for exhausted CD8 + T cells (CD8Tex) in breast cancer. Machine learning methods were used
In this study, single immune cell data sets were used to identify marker gene sets for exhausted CD8+T cells (CD8Tex) in breast cancer. Machine learning methods were used to cluster subtypes and establish the prognostic models with breast cancer bulk data using the gene sets to evaluate the ...
T cells can acquire a broad spectrum of differentiation states following activation. At the extreme ends of this continuum are short-lived cells equipped with effector machinery and more quiescent, long-lived cells with heightened proliferative potential
This CD39highCD8+T cell population is enriched for cells with the phenotypic and functional profile of terminal exhaustion. These findings provide a new marker of T cell exhaustion, and implicate the purinergic pathway in the regulation of T cell exhaustion. Chronic viral infection induces an ...
Here we show that an interleukin-4 fusion protein (Fc–IL-4), a typical type 2 cytokine, directly acts on CD8+ T cells and enriches functional terminally exhausted CD8+ T (CD8+ TTE) cells in the tumour. Consequently, Fc–IL-4 enhances antitumour efficacy of type 1 immunity-centric ...
Ex-vivo single or combinational antibody blockade of TIGIT and/or PD-L1 restored viral-specific CD8+ T cell effector responses. The frequency of TIGIT+ CD4+ T cells correlated with the CD4+ T cell total HIV DNA. These findings identify TIGIT as a novel marker of dysfunctional HIV-specific ...
CD8+ T cells correlated with parameters of HIV and SIV disease progression. TIGIT remained elevated despite viral suppression in those with either pharmacological antiretroviral control or immunologically in elite controllers. HIV and SIV-specific CD8+ T cells were dysfunctional and expressed high levels...
T cell exhaustion is a state of dysfunction observed in CD8+ T cells during chronic viral infection and cancer1–4. During chronic viral infections, exhausted CD8+ T cells progressively lose functional capabilities, such as cytokine production, cytotoxicity and proliferative capacity5,6. During the ...
Additionally, GC-infiltrating CD39hiCD8+ T cells were also identified for tumor-reactive T cells, as these cells expanded in vitro were able to recognize autologous tumor organoids and induced more tumor cell apoptosis than those of expanded their CD39int and CD39-CD8+ counterparts. Furthermore...
用于检测表面CAR的1A7抗14G2a独特型抗体在内部与Dylight488和/或650抗体标记试剂盒偶联。在一些ATACseq实验(图S6,B和C)和所有ChIP-seq实验中,CD8 + T细胞 通过使用抗CD4磁性选择珠的Miltenyi阴性选择分离,并遵循制造商的方案。 13、胞内细胞因子染色 将CAR-T细胞和143B细胞按1:1的E:T (Effector:Target)比例...