The amount of protein was estimated by Lowry method. Phytochemical screening was done with various successive extracts such as methanol, ethanol and petroleum ether for confirmation of the presence of alkaloids, tannins, flavonoids, steroids, glycosides and carbohydrates.Key words: Cocoa, proteins, ...
Preceding chapters have mainly described various methods for sampling the different developmental stages and age groups of Mosquito populations. This chapter is concerned with analysing the numerical changes that occur in population size during the life-
Commonly used dye-based protein determination methods rely on the reactivity of few amino acid residues—mainly tryptophan and tyrosine (12) in the case of the Lowry and BCA assays, or a hydrophilic/hydrophobic balance of the proteins in the case of Bradford reagent (13). Systematic errors of...
All four methods quantitated the largest aggregates four-to five-fold lower and the next largest at no better than half the Lowry ratings. Only GF and IGF quantitated the two fractions of smaller aggregate within 10% of the expected value. The presence of about 50% of the two larger ...
Lowry method is well established method for estimation of proteins. Collagen is most abundant protein present in different parts of body tissue. Tissue soluble collagen level varies in different clinical conditions like myocardial fibrosis, renal fibrosis etc. Hence, estimation of soluble collagen from ...
The method provides an accurate estimation of protein concentrations in the range of 5-40 micrograms, with a sensitivity similar to that of the original assay procedure. It yields hardly any protein-to-protein variation with simple proteins and eliminates the interference by four compounds that ...
In this study, the protein content in a laboratory GMP preparation and two commercial GMP samples, which was estimated by the Bradford, biuret, Lowry and bicinchoninic acid (BCA) assays, were compared using the Kjeldahl method. There were apparent differences in the values of estimated proteins ...
The most accurate method known to data for estimating age of wild vertebrates involves biochemical assay of eye lens proteins. Laboratory procedures that may be unfamiliar to many ecologists are described in detail. The method is based on precise changes in the amount of insoluble lens protein, ...
Nitrites are carcinogenic, especially when they are combined with other organic species, i.e., proteins, and produce nitrosamines giving rise to malignancies (e.g., ovarian cancer in females and other malignancies) [61]. The studies have proven that continuous exposure to heavy doses of ...