Tissues were prepared for immunohistochemical labeling of P2X7 receptor, HuC/D (Hu) (pan-neuronal marker) and S100β (glial marker); HuC/D (Hu) and glial fibrillary acidic protein (GFAP, glial marker)/DAPI (nuclear marker); or S100β and GFAP/DAPI. Qualitative and quantitative analyses of ...
Glial cells have been proposed as a source of neural progenitors, but the mechanisms underpinning the neurogenic potential of adult glia are not known. Using single cell transcriptomic profiling, we show that enteric glial cells represent a cell state attained by autonomic neural crest cells as they...
Enteric glial cells, strictly associated with enteric neurons to build up enteric ganglia, belong to the enteric nervous system (ENS), which coordinates many bowel functions. The ENS is spread throughout the gut in myenteric and submucosal plexuses. In our research work, we studied immunohistochemi...
The transcriptome of enteric glial cells (EGCs) was investigated using PLPCreERT2 Rpl22HA mice. In addition, we assessed the effect of supernatant from neurosphere-derived EGCs on monocyte differentiation based on the expression of pro- and anti-inflammatory factors. Results: Muscularis inflammation ...
Sox2 is a transcription factor known as a marker of ENS progenitor cells and is largely expressed by EGC in the adult animal [45, 46]. Thus, we used HuC/D+/Sox2+ double-labeling to evaluate the presence of Sox2-expressing neurons (Fig. 4), a putative marker of enteric neurogenesis [...
Whereas the contributions of epithelial2,3, immune4,5 and stromal cells6,7 in intestinal tissue integrity are well understood, the role of intrinsic neuroglia networks remains largely unknown. Here we uncover important roles of enteric glial cells (EGCs) in intestinal homeostasis, immunity and ...
for aquaporin-4 and for the glial marker glial fibrillary acidic protein verified that glial cells were not immunoreactive to aquaporin-4. We further confirmed our findings with additional aquaporin-4 antibodies and Western blot analysis. We found that, in addition to expressing aquaporin-4, the ...
Enteric glia differ from Schwann cells in that they do not form basal laminae and they ensheath axons, not individually, but in groups. Although enteric glia are rich in the S‐100 and glial fibrillary acidic proteins, it has been difficult to find a single chemical marker that distinguishes...
migration. This has mainly been studied in the context of perineural invasion (PNI), which in CRC, is associated with a reduced disease-free survival and can serve as an independent prognostic marker for patient outcome [288,289]. However, active crosstalk contributing to cancer growth, mostly ...
inflammatory bowel disease; IBD; TNBS-induced colitis; colonic dysmotility; angiotensin II; AT1 and AT2 receptors; nitric oxide; interstitial cells of Cajal; ICC; enteric glial cells; EGC1. Introduction Inflammatory bowel disease (IBD) includes Crohn’s disease (CD) and ulcerative colitis (UC). ...