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Molecular cloning, sequence analysis, and functionof the intestinal epithelial stem cell marker Bmi1in pig intestinal epithelial cells. Li CM,Yan HC,Fu HL, et al. Journal of Animal Sciences . 2014Li, C.M.; Yan, H.C.; Fu, H.L.; Xu, G.F.; Wang, X.Q. Molecular cloning, sequence...
Intestinal epithelial stem cells are present at the base of the crypts and give rise to transit-amplifying cells, which differentiate and mature into various cell types as they migrate towards the tip of the villi. These terminally differentiated cell-types, with the exception of Paneth cells, ...
Paneth cell marker (Lysozyme) (j), and goblet cell marker (Muc2) (k) in young mIOs treated with or without Amuc_1409*(8 nM). ChgA, Lysozyme, and Muc2 (green); DAPI (nuclei, blue). White scale bar, 50 μm. Yellow scale bar, 20 μm. Quantification data of positive cells ...
difficile induced inflammatory marker expression by human intestinal epithelial cells is asymmetrical. Life Sci. 2006, 78 (9): 920-925. 10.1016/j.lfs.2005.05.102. Article CAS PubMed Google Scholar Bambou JC, Giraud A, Menard S, Begue B, Rakotobe S, Heyman M, Taddei F, Cerf-Bensussan ...
undergo self-renewal, and generate all mature intestinal epithelial cell types, proving they are multipotent stem cells[12]. The CBC stem cells express several unique marker genes, such as Lgr5 and olfactomedin-4. Upon injury and stem cell loss, cells from a quiescent stem cell pool higher ...
Transepithelial Transport of Fluorescein in Caco-2 Cell Monolayers and Use of Such Transport in In Vitro Evaluation of Phenolic Acid Availability(Food & Nu... Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the ...
Understanding the development and function of an organ requires the characterization of all of its cell types. Traditional methods for visualizing and isolating subpopulations of cells are based on messenger RNA or protein expression of only a few known marker genes. The unequivocal identification of ...
(TNF)-α, IL-1β, IL-10 and IL-22 in colon tissue was significantly inhibited by A33+Li-EVs (Fig. 3f). Myeloperoxidase (MPO) is a marker for neutrophil and helps in assessing the neutrophil influx into inflamed tissue20. MPO activity can be used as a marker of inflammation. MPO ...
d Wild-type organoids were treated as indicated, and analyzed by in situ hybridization (RNAscope) using probes specific to Nfkbiz, Enpep (an enterocyte marker), Lyz1 (a Paneth cell marker), and Lgr5 (an intestinal stem cell marker). Magnified images of the indicated boxes are shown to ...