Therefore, the development of tools and techniques to better appraise enteric glia heterogeneity and plasticity will undoubtedly unveil critical regulatory mechanisms implicated in gut health and disease, as well as disorders of the gut-brain axis....
Glial cells have been proposed as a source of neural progenitors, but the mechanisms underpinning the neurogenic potential of adult glia are not known. Using single cell transcriptomic profiling, we show that enteric glial cells represent a cell state attained by autonomic neural crest cells as they...
CellBench: R/Bioconductor software for comparing single-cell RNA-seq analysis methods. Bioinformatics 36, 2288–2290 (2020). Finak, G. et al. MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data. Genome Biol....
Here, we investigate M heterogeneity at different stages of muscularis inflammation and determine how environmental cues can attract and activate tissue protective Ms. Design: Single cell RNA sequencing was performed on immune cells from the muscularis of wild-type and CCR2-/- mice at different ...
22 (PTPN22), interleukin-23 receptor (IL-23R), and interleukin-10 (IL-10) promoters related to immune cell regulation were reported to contribute to the heterogeneity of disease pathogenesis [79]. Another enteric neuropathy, gastroparesis, is characterized by delayed gastric emptying in the absence...
Siddique T, Figlewicz DA, Pericak-Vance MA, Haines JL, Rouleau G, Jeffers AJ, Sapp P, Hung WY, Bebout J, McKenna-Yasek D et al (1991) Linkage of a gene causing familial amyotrophic lateral sclerosis to chromosome 21 and evidence of genetic-locus heterogeneity. N Engl J Med 24(20):...
Molecular heterogeneity of quiescent melanocyte stem cells revealed by single-cell RNA-sequencing 2024, Pigment Cell and Melanoma Research Hirschsprung disease 2023, Nature Reviews Disease Primers Development of the Gastrointestinal Tract in Newborns as a Challenge for an Appropriate Nutrition: A Narrative...
Being among the most dynamic cells of the digestive system, EGCs react to cues in their surrounding microenvironment and communicate with various cell types and systems within the gut. Morphological studies and recent single cell RNA sequencing studies have unveiled heterogeneity ...
Our results suggest that neurons and EGCs show heterogeneity, and GFAP and NF-H cannot be used as panenteric glial or panneuronal markers, respectively. We also demonstrated, for the first time, the prenatal expression of S100A6 in enteric neurons and the possibility of using this protein for...
The use of a single-cell approach exploits cell heterogeneity and provides insight into the involved cellular changes of gene expression across the disease severity axis, allowing exploration of the molecular pathways, genes, and gene regulatory networks involved in specific disease states and cellular ...