焦磷酸香叶基香叶酯(GGPP)环合形成ent-焦磷酸古巴酯(ent-CPP)是穿心莲内酯生物合成的关键步骤,决定了穿心莲内酯的母核结构,而催化这个环合反应的二萜合酶——ent-柯巴基焦磷酸合酶(ent-copalyl diphosphate synthase,简称CPS),是影响穿心莲内酯生物合成的关键限速酶(图1)。Maison等[11]在对穿心莲(Andrographis ...
Here, we describe the functional characterization ofent-copalyl diphosphate synthase (SmCPSent), kaurene synthase (SmKS) and kaurene oxidase (SmKO) in the gibberellin (GA) biosynthetic pathway. SmCPSentcatalyzes the cyclization of GGPP toent-copalyl diphosphate (ent-CPP), which is converted toent-...
www.nature.com/scientificreports OPEN Functional characterization of ent- copalyl diphosphate synthase, kaurene synthase and kaurene received: 29 October 2015 oxidase in the Salvia miltiorrhizaaccepted:24February2016 Published:14March2016 gibberellin biosynthetic pathway Ping Su1,2,*,Yuru Tong1,2,*,...
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Here, we describe the functional characterization of ent-copalyl diphosphate synthase (SmCPSent), kaurene synthase (SmKS) and kaurene oxidase (SmKO) in the gibberellin (GA) biosynthetic pathway. SmCPSent catalyzes the cyclization of GGPP to ent-copalyl diphosphate (ent-CPP), which is converted to...