英文别名 ent-copalyldiphosphate,copalyldiphosphate 物化性质 密度 1.23g/cm3 沸点 563.4°Cat760mmHg 闪点 294.5°C 安全信息 符号词 信号词 危险说明 防范说明 限时折扣 N,N-二甲基甲酰胺二甲缩醛 CAS:4637-24-5 热销包装1 ￥ 2450 /25kg 热销包装2 ￥ 4850 /50kg N-甲基吡咯烷酮(NMP) CAS:872...
E,E)-geranylgeranyl diphosphate (GGPP; 1; see Scheme 1) intoent-copalyl diphosphate (ent-CPP; 2). This reaction is cata-lyzed by ent-CPP synthases (CPSs), which are ubiquitouslyfound in all vascular plants, thus providing a source forrepeated evolution of more specialized labdane-relatedditer...
Keeling CI, Dullat HK, Yuen M, Ralph SG, Jancsik S, Bohlmann J (2010) Identification and functional characterization of monofunctional ent -copalyl diphosphate and ent -kaurene synthases in white spruce reveal different patterns for diterpene synthase evolution for primary and secondary metabolism in...
Here, we describe the functional characterization ofent-copalyl diphosphate synthase (SmCPSent), kaurene synthase (SmKS) and kaurene oxidase (SmKO) in the gibberellin (GA) biosynthetic pathway. SmCPSentcatalyzes the cyclization of GGPP toent-copalyl diphosphate (ent-CPP), which is converted toent-...
SmCPSent catalyzes the cyclization of GGPP to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by SmKS. Then, SmKO catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid. Our results show that the fused enzyme SmKS-SmCPSent increases ent-kaurene ...
Novel product chemistry from mechanistic analysis of ent-copalyl diphosphate synthases from plant hormone biosynthesis. Angew. Chemie 53, 7198-7202.Potter, K., Criswell, J., Zi, J., Stubbs, A. & Peters, R. J. Novel product chemistry from mechanistic analysis of ent-copalyl diphosphate ...
An active-site water molecule coordinated by conserved histidine and asparagine residues seems to serve as the catalytic base in all ent-copalyl diphosphate synthases (CPSs). When these residues are substituted by alanine, the mutant CPSs produce stereochemical novel ent-8-hydroxy-CPP. Given the ...
Shen, Q., Li, L., Jiang, Y. & Wang, Q. Functional characterization of ent-copalyl diphosphate synthase from Andrographis paniculata with putative involvement in andrographolides biosynthesis. Biotechnology Letters 38, 131-137 (2016).Shen Q, Li L, Jiang Y, et al. Functional characterization of...
(2014) Novel product chemistry from mechanistic analysis of ent-copalyl diphosphate synthases from plant hormone biosynthesis. Angew. Chem. Int. Ed. (Engl.) 53, 7198-7202Potter K, Criswell J, Zi J, Stubbs A, Peters RJ (2014) Novel product chemistry from mechanistic analysis ...
Köksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase. Nat Chem Biol 2011, 7: 431–433.Koksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl...