Tetracyclic hydrocarbon ent-kaurene is a biosynthetic intermediate of GAs, and is converted from geranylgeranyl diphosphate, a common precursor of diterpenoids, via ent-copalyl diphosphate (ent-CDP) through successive cyclization reactions catalysed by two distinct diterpene synthases, ent-CDP synthase and...
Previous studies found four different diterpenoid biosynthetic pathways from the universal diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP) inS. miltiorrhiza. Here, we describe the functional characterization ofent-copalyl diphosphate synthase (SmCPSent), kaurene synthase (SmKS) and kaurene ...
www.nature.com/scientificreports OPEN Functional characterization of ent- copalyl diphosphate synthase, kaurene synthase and kaurene received: 29 October 2015 oxidase in the Salvia miltiorrhizaaccepted:24February2016 Published:14March2016 gibberellin biosynthetic pathway Ping Su1,2,*,Yuru Tong1,2,*,...
enzyme catalysis, terpene synthase, natural products, biosynthesis, hydratasesAn active site water molecule coordinated by conserved histidine and asparagine residues seems to serve as the catalytic base in all ent -copalyl diphosphate synthases (CPSs). When these residues are substituted by alanine, ...
produces ent-copalyl diphosphate (ent-CDP) and syn-CDP as precursors for several classes of phytoalexins and the phytohormones, gibberellins (GAs). It has recently been shown that a loss-of-function mutation of OsCPS1, a gene encoding a putative ent-CDP synthase, results in a severely GA-...
Shen, Q., Li, L., Jiang, Y. & Wang, Q. Functional characterization of ent-copalyl diphosphate synthase from Andrographis paniculata with putative involvement in andrographolides biosynthesis. Biotechnology Letters 38, 131-137 (2016).Shen Q, Li L, Jiang Y, et al. Functional characterization of...
Köksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase. Nat Chem Biol 2011, 7: 431–433.Koksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl...
Misra R C, Garg A, Roy S, Chanotiya C S, Vasudev P G, Ghosh S (2015) Involvement of an ent-copalyl diphosphate synthase in tissue-specific accumulation of specialized diterpenes in Andrographis paniculata Plant Sci 240 50- 64Misra RC, Garg A, Roy S, et al. Involvement of an ent- ...
( 2005 ) The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase . Plant Mol. Biol. 59 , 881 – 894 .Harris LJ, Saparno A, Johnston A, Prisic S, Xu M, Allard S, Kathiresan A, Ouellet T, Peters RJ. The maize An2 gene is induced by ...
In a lower land plant, the moss Physcomitrella patens, a single bifunctional diterpene synthase (diTPS) catalyzes both steps. In contrast, in angiosperms, the two consecutive cyclizations are catalyzed by two distinct monofunctional enzymes, ent-copalyl diphosphate synthase (CPS) and ent-kaurene ...