Here, we describe the functional characterization ofent-copalyl diphosphate synthase (SmCPSent), kaurene synthase (SmKS) and kaurene oxidase (SmKO) in the gibberellin (GA) biosynthetic pathway. SmCPSentcatalyzes the cyclization of GGPP toent-copalyl diphosphate (ent-CPP), which is converted toent-...
Köksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase. Nat Chem Biol 2011, 7: 431–433.Koksal M, Hu H, Coates RM, Peters RJ, Christianson DW: Structure and mechanism of the diterpene cyclase ent-copalyl...
et al. Functional characterization of ent -copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathway. Sci. Rep. 6, 23057; doi: 10.1038/srep23057 (2016). This work is licensed under a Creative Commons Attribution 4.0 ...
produces ent-copalyl diphosphate (ent-CDP) and syn-CDP as precursors for several classes of phytoalexins and the phytohormones, gibberellins (GAs). It has recently been shown that a loss-of-function mutation of OsCPS1, a gene encoding a putative ent-CDP synthase, results in a severely GA-...
Shen, Q., Li, L., Jiang, Y. & Wang, Q. Functional characterization of ent-copalyl diphosphate synthase from Andrographis paniculata with putative involvement in andrographolides biosynthesis. Biotechnology Letters 38, 131-137 (2016).Shen Q, Li L, Jiang Y, et al. Functional characterization of...
The structure of ent-copalyl diphosphate synthase reveals three ?-helical domains (?, ? and ?), as also observed in the related diterpene cyclase taxadiene synthase. However, active sites are located at the interface of the ?? domains in ent-copalyl diphosphate synthase but exclusively in the...
In silico saturation mutagenesisEnt-copalyl diphosphate synthase controls the biosynthesis of gibberellin plant hormones, which in turn coordinate the expression of numerous enzymes. Some gibberellin-dependent genes encode enzymes...doi:10.1007/s12539-019-00342-xPiotr Szymczyk...
Misra R C, Garg A, Roy S, Chanotiya C S, Vasudev P G, Ghosh S (2015) Involvement of an ent-copalyl diphosphate synthase in tissue-specific accumulation of specialized diterpenes in Andrographis paniculata Plant Sci 240 50- 64Misra RC, Garg A, Roy S, et al. Involvement of an ent- ...
Furthermore, the OsCPS2 transgene under regulation of the OsCPS1 promoter complemented the dwarf phenotype of an OsCPS1 mutant, oscps1-1. The results indicate that transcripts of the two ent-CDP synthase genes differentially localize in rice plants according to their distinct biological roles, Os...
Shen Q,Li Li,Jiang Y,et al. Functional characterization of ent- copalyldiphosphate synthase from Andrographis paniculata with putative involvement in andrographolides biosynthesis[J]. Bio- technol Lett,2016,38 (1 ) :131 .Shen, Q., Li, L., Jiang, Y. & Wang, Q. Functional characterization ...