Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector integration into the host cell genome (2). pLVX-EF1α-IRES-Puro contains all of the viral processing elements necessary for the ...
(MCS) and the mCherry gene. The IRES allows a protein of interest and mCherry to be translated from a single bicistronic mRNA. Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector...
Description: LV-EF1α-NLS-mCherry-Puro is a pre-made lentivirus which expresses mCherry fused with SV40 nuclear localization sequence (NLS) under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format. Titer: > 1x109 TU/mL Selection Antibiotic:...
Description: LV-EF1a-Mito-DsRed-IRES-Puro is a pre-made lentivirus which expresses Mito-EDsRed under the EF1a promoter wth the co-expression of the Puro resistance marker via the IRES element. The mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase (Mito) is fused...
note="Human elongation factor-1a promoter, forward primer" protein_bind 1440..1473 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN ...
promoterEF1a sequencing_primer_3GCATTAAAGCAGCGTATCCACATAGC sequencing_primer_5TTTTTGAGTTTGGATCTTGG site_3_destroyed site_5_destroyed entrez_gene genbank_ids mutation namemCherry shRNA_sequence size715 species 32644 Other Aequorea victoria tags ...
promoter sequencing_primer_3 sequencing_primer_5 vector_types AAV growth notes : These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources...
PromoterEF1a Tag/ Fusion Protein Nuclear localization signal (N terminal on insert) Cloning Information Cloning methodLigation Independent Cloning 5′ sequencing primerEF1a F1 3′ sequencing primerWPRE R1(Common Sequencing Primers) Resource Information Supplemental Documents pOTTC1032 - pAAV EF1a Nuc-flox...
The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean eukaryotic glyceraldehyde-3-phosphate dehydrogenasegene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive...
聚合酶离开启动子(启动子清除,promoter clearence),RNA链延伸。 σ70被NusA取代。 转录终止,NusA解离,RNA聚合酶循环使用。 2.1.6 转录终止 细菌中发现了两种转录终止模式:依赖于ρ因子的和不依赖于ρ因子的。 ρ因子具有ATP-dependent RNA-DNA helicase活性。依赖于ρ因子的转录终...