Moreover, the high-expression clones harbouring the EF-1伪 promoter tended to be more stable in long-term culture, even in the absence of selection pressure. According to these findings, we concluded that the EF-1伪 promoter is a potent regulatory sequence for episomal vectors because it ...
The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (Uetsuki et al. 1989; Wan Kim et al. 1990). More Cat. #ProductSizeLicenseQuantityDetails 631990 pLVX-EF1alpha-DsRed-Monome...
coli EM7 promoter. In mammalian cells, the resistance gene is transcribed from the rat EF-1α promoter as a polycistronic mRNA and translated via the FMDV IRES. • EF1 pAn is a strong polyadenylation signal. • MCS1 and MCS2: Each multiple cloning site contains several restriction sites ...
关键词: Animals Humans Cricetulus Cytomegalovirus CHO Cells Peptide Elongation Factor 1 Immediate-Early Proteins Genetic Vectors beta-N-Acetylhexosaminidases Promoter Regions, Genetic DOI: 10.1007/s10528-006-9016-3 被引量: 11 年份: 2006 收藏 引用 批量引用 报错 分享 ...
Remarkably, the toxicity did not stem from the heterologous expression of HIS3 (Schizosaccharomyces pombe his5+), but from the multi-copy introduction of the promoter driving this gene; the EF-1α promoter from Ashbya gossypii. This promoter is included in many ubiquitously used heterologous ...
The three genes follow each other immediately in the order EF-1α·S10·tRNAser after a putative promoter located 55 bp upstream of the EF-1α gene. Alignment of the derived EF-1α sequence with the corresponding sequences from Eukarya, Bacteria/organelles, and with available archaeal sequence...
Ligand-activated PPARβ efficiently represses the induction of LXR-dependent promoter activity through competition with RXR Angiopoietin-like protein 3 ( angptl3), a member of the vascular endothelial growth factor family, was shown to play an important role in regulating lipid ... K Matsusue,A ...
Previously, we showed that overexpression of the TEF-1-related factor (RTEF-1) increased the α1-adrenergic response of the skeletal muscle α-actin promoter, whereas TEF-1 overexpression did not. Here, we identified the functional domains and specific sequences in RTEF-1 that mediate the α...
Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of... Streptomyces coelicolor and Streptomyces pilosus produce desferrioxamine siderophores which are encoded by the desABCD gene cluster. S. pilosus...
Promoter(启动子):是一段位于结构基因5‘端上游区的DNA序列,能活化RNA聚合酶,使之与模板DNA准确地结合并具有转录起始的特异性。 satellite DNA:又称为随体。只在真核生物中发现的,占基因组的10%~60%,由6~100个碱基组成,在DNA链上串联重复成千上万次。