Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad...
Here, we show that this effect is due to the EF-1α promoter in the HIS3MX marker cassette rather than the tags per se. This promoter is frequently used in heterologous marker cassettes, including HIS3MX, KanMX, NatMX, PatMX and HphMX. Toxicity could be eliminated by promoter replacement...
21. AMICI C, ROSSI A, COSTANZO A, et al. Herpes simplex virus disrupts NF-kappa B regulation by blocking its recruitment on the IkappaBalpha promoter and directing the factor on viral genes[J]. J Biol Chem, 2006,281(11):7110-7117....
[8]Yang X, Hu C, Xing Z, et al. Prediction of Ki-67 labeling index, ATRX mutation, and MGMT promoter methylation status in IDH-mutant astrocytoma by morphological MRI, SWI, DWI, and DSC-PWI[J]. Eur Radiol, 2023,...
(rAAV) vector comprising a nucleic acid sequence encoding a normal retinal pigment specific epithelial 65 (RPE65) gene operably linked to a chicken beta actin promoter/CMV enhancer, wherein said rAAV vector is administered in a dosage of from 1×109to 2×1012rAAV vector in a volume of ...
3.2.1. 强启动子,加入CMV,chicken β-actin promoter加CMV enhancer; 3.2.2. 加入促进基因表达的内含子和转录后调控因子(WPRE); 3.2.3. 使用翻译效率更好的密码子Kozak系统; 3.3.4. 增加小RNA的表达,使用U6 small nuclear RNApromoter和H1 promoter等。但是一味的追求蛋白质和RNA的高表达对于宿主细胞来说也会...
Here we identified the gluconeogenic enzyme fructose-1,6-bisphosphatase 1 (FBP1) as a p53 target that is elevated in senescent-like MASH hepatocytes but suppressed through promoter hypermethylation and proteasomal degradation in most human HCCs. FBP1 first declines in metabolically stressed premalignant...
The present invention is directed to the identification of a novel repressor located between 1.2 kb to 1.6 kb from the translation start site of the IFN-λ1 promoter. The present invention provides a method of using siRNAs against ZEB1 (binds to the repressor region) and BLIMP-1 (binds out...
PU.1and a TTTAAA element in the myeloid Defensin-1 promoter create an operational TATA box that can impose cell specificity onto TFIID Function PU.1and a TTTAAA element in the myeloid Defensin-1 promoter create an operational TATA box that can impose cell specificity onto TFIID Function... ...
在人类基因组中,并非所有的序列均被编码,即是某种蛋白质的模板,已完成编码部分仅占人类基因总序列的3~5%,显然,手工的搜索如此大的基因序列是难以想象的.侦测密码区的方法包括测量密码区密码子(codon)的频率,一阶和二阶马尔可夫链,ORF(Open Reading Frames),启动子(promoter)识别...