EDC-NHS法偶联乳胶微球 Activation 1. Wash particles (e.g., 100 mg of 1 μm carboxylated latex beads) into coupling buffer (50 mM MES, pH 6.0). Suspend in 5 ml coupling buffer. The addition of a dilute detergent solution may be done to increase bead stability and prevent clumping (e....
Procedure for Using EDC for Coupling Haptens to a Carrier Protein Materials Required • Carrier protein: 2 mg bovine serum albumin (BSA), ovalbumin (OVA) or keyhole limpet hemocyanin (KLH) • Conjugation Buffer: 0.1 M MES (2-[N -morpholino]ethane sulfonic acid), pH 4.5-5 (Product ...
Although NHS or Sulfo-NHS is not required for carbodiimide reactions, their use greatly enhances coupling efficiency. Furthermore, using NHS or Sulfo-NHS makes it possible to perform a two-step reaction. Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, ...
NHSisnotrequiredforcarbodiimidereactions,theirusegreatlyenhancescouplingefficiency.Furthermore,usingNHSor Sulfo-NHSmakesitpossibletoperformatwo-stepreaction. BothNHSandSulfo-NHSaresolubleinaqueousandorganicsolvents.ActivationwithNHS,however,decreaseswater-
Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS-EDC coupling. Cellulose 2014, 21, 4209-4218.Arcot, L. R.; Lundahl, M.; Rojas, O. J.; Laine, J. Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS- EDC coupling. Cellulose 2014, 21, 4209...
EDC-NHS ARTICLE IN PRESS +Model Journal of Molecular Catalysis B:Enzymatic 45(2007)84–90 Covalent binding of ␣-chymotrypsin on the magnetic nanogels covered by amino groups Jun Hong a ,b ,Dongmei Xu a ,b ,Peijun Gong a ,b ,Hanwen Sun a ,b ,Li Dong a ,b ,Side Yao a ,∗ a...
EDC和NHS与sulfo-NHS使用说明
在存在 N-羟基磺基琥珀酰亚胺(磺基-NHS)的情况下,EDC 可用于将羧基转换为胺反应性磺基-NHS 酯。这是通过将 EDC 与含有羧基的分子混合并添加磺基-NHS 来实现的。应用:• 偶联肽和蛋白间的羧基与氨基基团• 偶联半抗原与免疫原性载体蛋白(如将肽连接至 KLH)• 将肽抗原固定在亲和纯化抗体上• 生成 ...
so what else could be coupling to the nanoparticles? Does EDC degrade to form any amines capable of reacting with activated NHS ester? The EDC I used was ~4 years old so my first thought was that it may have degraded but I wanted to get your opinions first. Any advice appreciated!
carbodiimide- mediated coupling reactions 其实就自己的试验来看 1：1 或者1：0.5的摩尔比是通用的 ...