Procedure for Using EDC for Coupling Haptens to a Carrier Protein Materials Required • Carrier protein: 2 mg bovine serum albumin (BSA), ovalbumin (OVA) or keyhole limpet hemocyanin (KLH) • Conjugation Buffer: 0.1 M MES (2-[N -morpholino]ethane sulfonic acid), pH 4.5-5 (Product ...
EDC-NHS法偶联乳胶微球EDC-NHS法偶联乳胶微球 Activation 1. Wash particles (e.g., 100 mg of 1 μm carboxylated latex beads) into coupling buffer (50 mM MES, pH 6.0). Suspend in 5 ml coupling buffer. The addition of a dilute detergent solution may be done to increase bead stability and ...
中间体在水性溶液中不稳定,因此两步偶联程序依赖于 N-羟基琥珀酰亚胺 (NHS) 进行稳定。如果不与胺发生反应,将导致中间体的水解、羧基的再生和 N-取代尿素的释放。副反应是形成 N-酰脲,通常仅限于位于蛋白疏水性区域的羧基。 Thermo Scientific No-Weigh 产品是可提供预分装规格的特殊试剂。预称重的包装可消除...
Although NHS or Sulfo-NHS is not required for carbodiimide reactions, their use greatly enhances coupling efficiency. Furthermore, using NHS or Sulfo-NHS makes it possible to perform a two-step reaction. Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, ...
NHSisnotrequiredforcarbodiimidereactions,theirusegreatlyenhancescouplingefficiency.Furthermore,usingNHSor Sulfo-NHSmakesitpossibletoperformatwo-stepreaction. BothNHSandSulfo-NHSaresolubleinaqueousandorganicsolvents.ActivationwithNHS,however,decreaseswater-
so what else could be coupling to the nanoparticles? Does EDC degrade to form any amines capable of reacting with activated NHS ester? The EDC I used was ~4 years old so my first thought was that it may have degraded but I wanted to get your opinions first. Any advice appreciated!
Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS-EDC coupling. Cellulose 2014, 21, 4209-4218.Arcot, L. R.; Lundahl, M.; Rojas, O. J.; Laine, J. Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS- EDC coupling. Cellulose 2014, 21, 4209...
EDC-NHS ARTICLE IN PRESS +Model Journal of Molecular Catalysis B:Enzymatic 45(2007)84–90 Covalent binding of ␣-chymotrypsin on the magnetic nanogels covered by amino groups Jun Hong a ,b ,Dongmei Xu a ,b ,Peijun Gong a ,b ,Hanwen Sun a ,b ,Li Dong a ,b ,Side Yao a ,∗ a...
It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds. Additionally, EDC can also be used to activate phosphate groups in order to form phosphomono-esters and phosphodiesters. Common uses...
EDC和NHS与sulfo-NHS使用说明