Procedure for Using EDC for Coupling Haptens to a Carrier Protein Materials Required • Carrier protein: 2 mg bovine serum albumin (BSA), ovalbumin (OVA) or keyhole limpet hemocyanin (KLH) • Conjugation Buffer: 0.1 M MES (2-[N -morpholino]ethane sulfonic acid), pH 4.5-5 (Product ...
Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS-EDC coupling. Cellulose 2014, 21, 4209-4218.Arcot, L. R.; Lundahl, M.; Rojas, O. J.; Laine, J. Asymmetric cellulose nanocrystals: thiolation of reducing end groups via NHS- EDC coupling. Cellulose 2014, 21, 4209...
ProcedureforTwo-stepCouplingofProteinsUsingEDCandNHSorSulfo-NHS Thefollowingprotocol,adaptedfromaproceduredescribedbyGrabarekandGergely,allowssequentialcouplingoftwo proteinswithout affecting the second protein ’s carboxyls by exposing them to EDC. This procedure requires quenching the ...
This results in higher coupling effi- ciencies. For more information regarding EDC coupling procedures, visit www.covachem.com and download our technical bulletins. Protocol for EDC coupling with Sulfo-NHS Protocol for EDC coupling with N-Hydroxysuccinimide Product Description Package Size and Product ...
or other nanoparticals. EDC-HCl is water soluble, thus the reaction can take place at physiological conditions; although the best results take place at pH of 5.5 or less buffered inMES.Sulfo-NHSorNHSis used as a catalyst for EDC-HCl coupling and greatly increases the efficiency of the react...
conversion of carboxyl groups to amine-reactive NHS esters. Largely used as activator to mediate EDC reaction on carboxyls.Introduction•Improves the yield of EDC-mediated amidation/coupling •Modify carboxyl in amines-reactive (sulfo)NHS esters •Sulfo-NHS derivatives are water soluble, allowing...
ZnO NPs have a very high dissolution rate under acidic conditions of EDC/NHS coupling chemistry as determined from the ICP-MS analysis. In addition, the amount of ZnO NPs in coupling reaction has an important effect on the dissolution rate of Zn2+and dependently BSA attached on the ZnO NP ...
2003. Pre- paration of multilayer plasma protein films on silicon by EDC/NHS coupling chemistry. Coll Surf B: Biointerfaces 28:261 - 272.P. Tengvall,E. Jansson,A. Askendal,P. Thomsen,C. Gretzer.Preparation of Multilayer Plasma Protein Films on Silicon by EDC/NHS Coupling Chemistry. ...
Kishida, "Controlling coupling reaction of EDC and NHS for preparation of collagen gels using ethanol/water co- solvents," Macromolecular Bioscience, vol. 8, pp. 32, 2008.Nam, K., Kimura, T., & Kishida, A. (2008). Controlling coupling reaction of edc and nhs for preparation of collagen...
Fischer MJ (2010) Amine coupling through EDC/NHS: a practical approach. Methods Mol Biol (Clifton, NJ) 627:55-73. doi:10. 1007/978-1-60761-670-2_3Fischer MJE. Amine coupling through EDC/NHS: a practical approach. In: Surface Plasmon Resonance: Methods and Protocols. JN Mol and MJE ...