• 使用胺反应性 Sulfo-NHS 酯活化纳米微粒 • 通过 5' 磷酸基进行 DNA 标记(参见 Tech Tip #30) 产品参考文献: 交联剂应用指南—检索本产品的近期参考文献 仅供科研使用。不可用于诊断程序。 规格 化学反应性胺基 - 羧基 可裂解否 描述EDC 分子量191.7 ...
2. Add 100 mg of EDC and 100 mg of sulfo-NHS. Mix to dissolve. To facilitate faster dissolution, EDC and sulfo-NHS may be dissolved immediately before use as a concentrated stock solution in reaction buffer and then an aliquot of this solution added to the particle suspension to obtain th...
Procedure for Using EDC for Coupling Haptens to a Carrier Protein Materials Required • Carrier protein: 2 mg bovine serum albumin (BSA), ovalbumin (OVA) or keyhole limpet hemocyanin (KLH) • Conjugation Buffer: 0.1 M MES (2-[N -morpholino]ethane sulfonic acid), pH 4.5-5 (Product ...
NHSisnotrequiredforcarbodiimidereactions,theirusegreatlyenhancescouplingefficiency.Furthermore,usingNHSor Sulfo-NHSmakesitpossibletoperformatwo-stepreaction. BothNHSandSulfo-NHSaresolubleinaqueousandorganicsolvents.ActivationwithNHS,however,decreaseswater-
Controlling Coupling Reaction of EDC and NHS for Preparation of Collagen Gels Using Ethanol/Water Co-Solvent. Macromol. Biosci. 2007;8:32-7.Nam K, Kimura T, Kishida A: Controlling coupling reaction of EDC and NHS for preparation of type-I collagen gels using ethanol/water co-solvents. ...
Although NHS or Sulfo-NHS is not required for carbodiimide reactions, their use greatly enhances coupling efficiency. Furthermore, using NHS or Sulfo-NHS makes it possible to perform a two-step reaction. Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, ...
EDC和NHS与sulfo-NHS使用说明
Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS By combining EDC and Sulfo-NHS amine reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule Amide bonds formed with this crosslinking reaction provide a neutral linkage ...
The above reaction yields and percentage compositions were estimated by fitting the carbonyl stretching region with 5 possible species, NHS-ester, anhydride, N-acylurea, unreacted acid, unhydrolyzed tert-butyl ester, and using the Beer–Lambert law. The different surface chemistry mechanisms will ...
other nanoparticals. EDC-HCl is water soluble, thus the reaction can take place at physiological conditions; although the best results take place at pH of 5.5 or less buffered inMES.Sulfo-NHSorNHSis used as a catalyst for EDC-HCl coupling and greatly increases the efficiency of the reaction...