针对ECM刚度的潜在抗癌策略包括预防/逆转硬化过程和破坏癌细胞对ECM机械信号的反应。然而,每种策略都有其局限性。 2024年12月20日,浙江大学梁霄,徐俊杰,Shangguan Liqing共同通讯在Advanced Science在线发表题为“Synergistic Anticancer Strategy Targeting ECM Stiffness: Integration of Matrix Softening and Mechanical Signa...
Using multi-omics analysis, we found that a decrease in ECM stiffness impeded the sorting of vesicular transport-related proteins and autophagy-related lipids into MSC-EVs, impairing their secretion and subsequent uptake by macrophages. Hence, MSC-EVs with different secretion and uptake behaviors can...
Figure 1.Impact of ECM stiffness on mitochondrial calcium influx.(A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), po...
细胞外基质(ECM)可以通过局部机械性能的改变影响基因的转录表达,从而调节干细胞的分化。比如,各种类型的干细胞和祖细胞被证明有一种感知外基质的刚性(stiffness)的能力。这些干细胞的形态、细胞骨架和迁移速率都在数小时内对ECM机械性能作出反应,而对增殖和或分化的影响则在数天内发生。 为了系统性测试MSCs的机械敏感性...
stiffness. Young chondrocyte cells looked old when put on a stiff surface due to the loss of Klotho, but when the researchers protected the cells from the stiffness in their environment, they observed improved chondrocyte health. “we discovered that increased matrix stiffness drove Klotho promoter ...
而调节ECM刚度( stiffness)的方法之一是使用不同弹性的水凝胶(如聚丙烯酰胺凝胶),通过改变凝胶的浓度达到调节ECM刚度的目的。体外研究的结果表明,干细胞可对不同ECM的刚度产生应答,并以此信号确定其分化方向。例如,生长在软凝胶中的ES细胞,即使在缺少白血病抑制因子( LIF)的情况下,仍可保持较高的自我更新能力和高...
ECM stiffness ROCK-myosin IIA-F-actin axis cGAS signaling TRIM14 protein tumor immunity Research topic(s) CP: Cancer CP: Immunology Introduction The tumor microenvironment (TME) is a complex ecosystem comprising an altered extracellular matrix (ECM) encasing tumor cells and various stromal cells (...
硬度(stiffness):物体或流体的硬度。胞外基质的硬度对于引导细胞的趋硬性(durotaxis)迁移是重要的。硬度可以帕斯卡(pa)为单位,即每平方米一牛顿。 治疗剂:以一般意义使用,其包括治疗剂、预防剂和替代剂。“治疗(treatment)”或”治疗(treating)”是指向患者提供足以可测量地减少、抑制或减轻任何疾病症状,减缓疾病进展...
Active Manipulation of ECM Stiffness and Its Effect on Endothelial Cell Migration during Angiogenesis We have developed an engineering approach for actively manipulating the local stiffness of the extracellular matrix (ECM) and studied its effect on the migration of endothelial cells (ECs). In this ...
Stem cells mechanosense the stiffness of their microenvironment, which impacts differentiation. Although tissue hydration anti-correlates with stiffness, extracellular matrix (ECM) stiffness is clearly transduced into gene expression via... LR Smith,C Sangkyun,DE Discher - 《Physiology》 被引量: 7发表...