Quantitative proteomics of exosomes combined with genomic analysis of human breast tumor tissues (TCGA database) identified thrombospondin-1 (THBS1) as a prospective regulator of stiffness-dependent cancer invasion. Knockdown studies confirmed that the pro-invasive effects of stiffness-tuned exosomes are ...
1g). CoQ10 does increase stiffness in MCF7, MDA-MB-468, and MDA-MB-231, but not in non-tumorigenic MCF10A suggesting that the effect of CoQ10 is possibly related to the tumorigenic state of breast cancer cells or rather a different PM composition. To further confirm the ability of CoQ1...
and XI) also has direct implications for metastatic dissemination [23]; for example, increased collagen density elevates vascular basement membrane tension, augmenting its permeability to cancer cells [23,24] and high matrix stiffness enhances the adhesion of metastatic cancer cells to the tumor endot...
Figure 1.Impact of ECM stiffness on mitochondrial calcium influx.(A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), po...
and remodeling of ECM [85]. During tumor progression, stromal EMT deposition increases, altering the chemical composition and mechanical properties of ECM [86]. Cancer cells' invasive capabilities are further enhanced by the mechanical stretching of the collagen matrix or increased matrix stiffness [...
2018). Current medications for OA are symptom-based and can provide temporary relief from the disease, mainly focusing on alleviating pain and joint stiffness, while maintaining physical joint function. Nonetheless, they have many side effects (Latourte et al. 2020). Thus, there is an urgent ...
Finally, we discuss the importance of this sensing for major cell functions by taking cell migration and cancer as examples. Show more 0 comments Cited 467 times –based on 0 reviews Review now Bookmark Record: found Abstract: not found Article: not found The stiffness of living tissues and...
[2] Buchheit C L,Weigel K J,Schafer Z T.Cancer cell survival dur-ing detachment from the ECM:multiple barriers to tumour progres-sion[J].Nat Rev Cancer,2014,14(9):632-41. [3] Pathak A,Kumar S.Independent regulation of tumor cell migra-tion by matrix stiffness and confinement[J].Pro...
Recent studies have indicated that the remodeling of ECM is associated not only with proliferation but also with cellular dormancy and can impact tumor cell dormancy through altering environmental stiffness [[10], [11], [12]]. Yuki Ohta et al. discovered that COL17A1 alters the dormant state ...
During tumour development, cells within the tumour microenvironment (TME), such as tumour cells and cancer associated fibroblasts (CAFs) promote ECM stiffness resulting in fluid and solid stress in the TME. This, in turn, results in activation of mechanotransduction pathways [3,4] which drive chan...