D, quantification of DNA content and cell cycle phase distribution in empty vector- and DOC2B-transfected SiHa cells at different time points as analyzed by a BrdU pulse-chase experiment. Ectopic expression of the DOC2B gene resulted in a significant cell cycle arrest at G0/G1 and S phases ...
was specifically associated with nascent DNA we used thymidine chase experiments as previously described41using the same set-up. As expected, the levels of PLA foci specific for histone H1-EdU were not altered at any time point following thymidine chase whereas the levels of PLA foci specific for...
This conclusion was based on bulk biochemical experiments, in which Dcr-2 was allowed to cleave radiolabeled dsRNAs for a short time and then challenged by a vast excess of cold dsRNAs. However, such a pulse-chase assay cannot formally distinguish whether the dissociation of Dcr-2 from ...
Antetokounmpo banged his finger on the rim while trying to chase down RJ Barrett’s fast-break dunk late in the second quarter. He finished with 11 points, 13 assists and 12 rebounds in a128-104 road winover the struggling Raptors. “I’m fine,” Antetokounmpo said...
The distance between the two doppler probes was measured using a scientific caliper, and WinDAQ Waveform Browser (DataQ Instruments) was used to determine the transit time of pulse waveform between the doppler probes, using the foot-to-foot method as described previously [19, 21]. Distance ...
The flow field and separation efficiency of the double spherical tangent and the straight double-cone coupling device are analyzed, and confirmatory experiments are carried out. The results show that the numerical model can reasonably predict the separation performance of the coupling device. The ...
Using this approach in a flow cytometry-based pulse chase assay, we observed a decrease in post-pemetrexed treatment IdU incorporation in UNG À / À cells (Figure 6b) While this is consistent with decreased replication fork progression in pemetrexed-treated UNG À / À cells, these data...