ELISA in tuberculosis meningitis: using two mycobacterial antigens does not improve the diagnostic yield compared to either antigen alone. Serodiag Immunother Infect Dis 1990; 4: 173-181.AI-Ramahi SM, Araj GF, Chugh TD, Shakir RA: ELISA in tuberculous meningitis: using two mycobacterial antigen...
Why inhibition of MMP-9 with monoclonal antibodies improves DSS-induced colitis9,10, whereas inhibition with small peptides does not, is puzzling. To rule out that insufficient levels were attained, we measured the levels of peptide inhibitors A and B in plasma for all three administration ...
Serum samples from weeks 4, 6, 8, 10, 13, 15, 18, and 22 were tested by microsphere immunoassay (MIA) to detect anti-SU and/or anti-CD134 IgG antibodies in cats vaccinated with FIV-PPR-SU-huFc or a combination of CD134+SU-huFc; results of which are presented in Fig.2. Increas...
Fig. 5. ELISA quantification of serum and stool anti-rotavirus and total serum antibodies following rotavirus (EDIM) challenge. Unvaccinated control diet (RBD−, RRV−, solid black circles) and unvaccinated regional basic diet (RBD−, RRV−, solid grey triangles) weanling mice were vaccinat...
Serology is another method used to identify HPV infection, by detecting antibodies to HPV capsid proteins in blood samples. One advantage of serology is that it can detect any prior exposure to HPV since antibodies can persist for a lifetime, even after the clearance of HPV DNA [81]. However...
Secondary antibodies, goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor™ 555 (dilution 1:200, Thermo Scientific, cat. no. A-21428), and goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor™ 488 (dilution 1:200, Thermo Scientific, cat. ...
: Immunofluorescence (IF) is a technique to detect specific target antigens in nasal or nasopharyngeal swab samples or in frozen (cryostat) sections from aborted fetal tissues (lung, liver, thymus and spleen) and placental tissue in the detection of EHV using the fluorescent-labelled antibodies....
AccuDiag™ CA15-3 ELISA Kit from Diagnostic Automation/Cortez Diagnostics Inc., Calabasas, CA, USA, was used for this study. This ELISA test is a two-site solid-phase enzyme immunoassay. The molecules of CA15-3 are “sandwiched” between two monoclonal antibodies – one coated to the botto...
serum electrophoresis, the antibody levels in a quantitative ELISA for the detection ofL. infantum-specific antibodies, the result forLeishmaniaqPCR andL. infantumIFN-γ concentration. Qualitative variables of healthy and sick dogs were assessed using Chi-quare, Fisher’s exact test, Kruskal-Wallis...
When dam status was defined by a combination of both PCR outcomes (faeces and colostrum) and the ELISA outcome the HR was 1.26 (CI 0.9; 1.9). Therefore, this study indicates that neither the presence of MAP DNA in colostrum, MAP DNA in faeces nor the presence of MAP antibodies in ...