Sieving study of chromatin and histone-DNA complex by porous hollow fiber membranes. Journal of Membrane Science 126: 7-17.A. Higuchi, T. Yoshimura, K. Kato, M. Hara, M. Higuchi, N. Minoura, T. Hirasaki, T. Sato, G. Ishikawa, H. Nakano, S. Satoh, S. Manabe, Sieving study of ...
Histone acetyl transferases(HATs) in a complex with theircofactor(acetyl-coenzyme A) catalyze the lysine acetylation that neutralizes the basic charge of tight DNA-histone interactions. In contrast, histone deacetylases (HDACs) mediate the removal of the acetylation mark, resulting in the formation ...
The effect of a given PTM on the DNA accessibility is likely to be more complex than simple partial unpeeling of the DNA from the intact histone core, since partially assembled states of the nucleosome, which can expose large regions of the DNA through removal of some of the core histones,...
It appears that the formation of the nonconservative spectrum which can be ascribed to a certain structural form of DNA is caused by orientation of the DNA molecules in the aggregated complex and by specific interactions of the bases of the guanine-cytosine pair. The possible mechanism of ...
The binding stoichiometry was estimated by DNA thermal melting and by fluorescence titration to be about 70 base pairs of DNA per PLP molecule; thus, since the overall charge on PLP is about 140, the saturated complex should be essentially charge neutral. In order to decrease problems of ...
Although the field of genetics has grown by leaps and bounds within the last decade due to the completion and availability of the human genome sequence, transcriptional regulation still cannot be explained solely by an individual's DNA sequence. Complex coordination and communication between a ...
How the FANCM- MHF complex recognizes branched DNA is unclear. Here, we solved the crystal structure of MHF and its complex with the MHF-interaction domain (referred to as MID) of FANCM, and performed structure-guided mutagenesis. We found that the MID-MHF complex consists of one histone H3...
Cryo-EM analysis of yeast endogenous replisome complex containing parental histones. A–C: Cylindrical atomic models showing cryo-EM views of three representative replisome complexes from different viewing angles with stably associated FACT-histone complexes. MCM subunits, Ctf4, Tof1, Csm3, Mrc1, Spt...
deposition是沉积的意思,但是在句子里说不通,本人刚刚开始看英文文献, 原句是In DNA methylation-rich regions,CAF1 forms a complex with methyl CpG-binding protein 1 (MBD1) and the Lys methyltransferase SETDB1 (also known as KMT1E),thereby coupling histone deposition with histone methylation. 2DNA...
Although histone lysine methylation is conserved among eukaryotes, the evolution of DNA methylation is more complex (reviewed in detail by Iyer et al. [8]), being found in some fungi (not including yeast), heterolobosea and stramenopiles, most plants, and some metazoans (including vertebrates,...