Efficient transcription of a DNA template associated with histone (H3.H4)2 tetramers.DNA-Directed RNA PolymerasesHistonesViral ProteinsDNAMicroscopy, ElectronTranscription, GeneticTemplates, GeneticA histone-DNA transcription template has been assembled, by dialysis against decreasing salt concentrations, from...
We have examined, by protein binding assays, thermal denaturation, and circular dichroism, the possible effects of histones on nonhistone chromosomal protein (NHCP) interactions with DNA. For these studies, we have fractionated mouse Krebs II chromosomal proteins into three discrete fractions: Mo, 5...
As they always associate with DNA, histones are highly possible candidate DPCs that block RNAPII progression39,40. To investigate whether histone-DPCs are targets of the TCR pathway, we developed an optional DPC-seq procedure to detect DNA-histone conjugates and measure their distribution. We ...
Strikingly, several of the cross-linked proteins we identified are associated with histones and nucleosome modification, such as BPTF, PHF19, and EP400. When we added adenine and guanosine adducts to the database search, we identified 15 additional proteins cross-linked to DNA, e.g., the ...
Linker histones of the H1 family are among the most abundant components of chromatin. In this issue of Cell, Fan et al. (2005) examine the effect of H1 dow... RAW Rupp,PB Becker - 《Cell》 被引量: 67发表: 2005年 Identification and analysis of the promoter region of the human DHCR24...
The pattern of products generated by cross-linking the polypeptides with dimethylsuberimidate is very similar to the pattern generated by cross-linking native core particles. The results indicate that histones which lack their HN2-terminal regions retain both the features necessary for correct protein-...
Also, The association of DNA with histones and other proteins introduces supercoiling that requires relaxation by topoisomerases. 1.1 Classification of DNA topoisomerase DNA topoisomerase is found in both prokaryotic and eukaryotic cells. In eukaryotic cell, DNA topoisomerase is further classified into ...
5. Li, Z., Hua, X., Serra-Cardona, A., Xu, X., Gan, S., Zhou, H., Yang, W.S., Chen, C.L., Xu, R.M., and Zhang, Z. (2020). DNA polymerase alpha interacts with H3-H4 and facilitates the tran...
Conformational studies of membrane proteins lag far behind those of soluble proteins mainly because of the difficulties associated with crystallization of membrane proteins for x-ray diffraction studies, and because of the restricted movement of the proteins embedded in the membrane for NMR studies (Küh...
However, DNA replication is generally associated with an increased level of histones in eukaryotic cells (reviewed in [45]). The mechanisms employed by P. falciparum to organize its genome-wide nucleosome eviction remain to be fully described. The P. falciparum genome is known to encode a ...