Function : Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair...
When the hybrid nanospheres were internalized into cells, they would be dissolved and liberated Y-shaped DNA probes to recognize TK1 mRNA via hybridization with the loop region of Y-DNA, resulting in red fluorescence. Interestingly, when biscyanine molecules were encapsulated in the DNA nanospheres ...
RemarksMother; donor subject has a single bp (G-to-A) transition at nucleotide 681 in exon 5 of the CYP2C19 gene (CYP2C19*2) which creates an aberrant splice site. The change altered the reading frame of the mRNA starting with amino acid 215 and produced a premature stop codon 20 amino...
It is currently unknown whether Dnmt3a2 mRNA will be immediately translated, as expected for an immediate early gene. In this case, it may not replace DNMT3A1 but independently facilitate activity-dependent gene expression. Funding and disclosure The authors have no conflict of interest to declare...
3.3. SERINC5 Downregulates HIV Gene Expression at the mRNA Level Since the presence of SERINC5 causes defects in lentivirus protein expression, we examined whether SERINC5 drives the degradation of these proteins. To test this hypothesis, fluctuations in HIV protein levels were evaluated in the pres...
(A) Bone marrow cells fromTrex1−/−mice were treated with aspirin at the indicated concentrations for 2 days. The mRNA levels of ISGs, as indicated, were analyzed by qPCR (relative to that of WT bone marrow cells). The IC50was calculated by Statistical Package for the Social Sciences ...
First-strand cDNA was synthesized by incubating 3 mg isolated mRNA, 50 ng random hexamers and 0.25 mM of each deoxynucleotide triphosphate at 65 oC for 5 min. After chilling, NATURE COMMUNICATIONS | 4:1423 | DOI: 10.1038/ncomms2395 | www.nature.com/naturecommunications & 2013 Macmillan ...
to Cd toxicity. However, the mRNA expression of GSH2 decreased, while its protein abundance did not change significantly under Cd exposure. It has been proposed that GSH1 and GSH2 display different sensitivity to Cd exposure and are induced at different Cd concentrations52. Also, the decreased ...
Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA...
RT-PCR was used to detect the E7 mRNA transcripts resulting from the E7 gene transcription encoded in the PEI/mcDNA and R8-mannose/PEI/mcDNA system vector. After 24 h of transfection, cells were lysed through the addition of TripleXtractor and incubated at room temperature for 5 min. Subseq...