Protein Mass - Moles Converter OD260to Concentration Mass Calculator from Molarity Dilution Calculator OD600Calculator Database ORF Search How to Use Vector Design Studio About VectorBuilder DNA Translation Input your DNA sequence below to retrieve the translated amino acid sequence. ...
Finally, the buffer of the ComK-containing fractions was exchanged to 0.5 M l-Arg pH 7.3 using a HiTrap desalting column (5 ml, GE). The protein concentration was determined using an extinction coefficient of Ɛ280 = 24,660 M−1 cm−1 computed from the amino acid ...
to lysine residues, interrupts degradation of DNMT3A1. This results in deficits in promoter methylation of activity-dependent genes, as well as synaptic plasticity and memory formation. In turn, the underlying molecular pathway is triggered by the induction of synaptic plasticity and in response to ...
aInterestingly, these soybean plants also accumulated increased levels of methionine, lysine, and isoleucine resulting in an upto 3.5-fold increase in the total free amino acid content; 有趣的是,氨基甲硫基丁酸、赖氨酸和异白氨酸的这些大豆植物也积累增加的水平造成3.5折叠在总游离氨基酸内容的增量; [...
The effect of dietary amino acid levels on growth performance and DNA methylation contents in tissues of broilers at different day-ages was studied.The Results showed: ① The dietary Met and Lys significantly influenced the growth performance and carcass characteristics in the broilers.② The DNA me...
Requirement 1: isolate the recombinant DNA structure, which is characterized by the separation of a polynucleotide from the group, and a supplement to the nucleoside sequence composed of (a) the first nucleoside sequence2. Two kinds of coding polypeptides, including the amino acid sequence of SEQ...
The ionic current was sampled at 100 kHz using a Digidata 1440A analog-to-digital converter (Molecular Devices) and processed with pClamp11 software (Molecular Devices). The current blockade represents the capture and the translocation of a probe DNA in the nanopore and is defined as I/I0 ...
The experiment was carried out on 864 one-day-old Hybrid Converter turkeys divided into six experimental groups (8 replicates per group and 18 birds per replicate) receiving different levels of Arg and Met. Chickens from group Arg90Met30 received 90% Arg and 30% Met relative to Lys; Arg90...
Molecular engineering seeks to create functional entities for modular use in the bottom-up design of nanoassemblies that can perform complex tasks. Such systems require fuel-consuming nanomotors that can actively drive downstream passive followers. Most
The lysates were sonicated using a digital sonifier (102C CE Converter; Branson). Proteins were separated by SDS–PAGE and transferred to a nitrocellulose membrane. Blocking and blotting with primary antibodies were performed in PBS-T supplemented with 5% skimmed milk powder. Proteins were visualized...