Each box in a promoter contains an adenine–thymine (AT)-rich sequence (Fig. 1d). Such A-tracts are ubiquitous throughout all kingdoms of life, presumably due to their potential to curve DNA32,33. To assess the structural properties of the promoter, we engineered a set of comG promoters...
While most TFs functioned exclusively as antagonists or antagonized TFs in our screen, 27 TFs participated in each role at different promoters, suggesting that the role of a given TF depends on its TF partner as well as the target DNA sequence (Fig. 3h). This is likely due to differences ...
DNA The FASTA file to be analyzed. --amino=<aminoAcid> The amino acid representing the start of a gene. --find=<proteinFile> The DNA sequence to be found within the FASTA file. -h, --help Show this help message and exit. --max=<maxCount> The maximum count of the reading frame....
(H) acetylated peptides of cGAS with acetyl-d3were analyzed by Thermo Scientific Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer. The y ion peaks are shown in blue, and the b ion peaks are shown in red. The partial amino acid sequence (from left to right, C-terminal to N-...
Previously, we conducted RNA sequence (RNA-seq) analysis under zinc (Zn), Cd, Cu, mercury (Hg) and lead (Pb) exposure in the Pacific oysters Crassostrea gigas. Although the results were submitted to the US National Center for Biotechnology Information (NCBI) database, detailed data analysis ...
Requirement 1: isolate the recombinant DNA structure, which is characterized by the separation of a polynucleotide from the group, and a supplement to the nucleoside sequence composed of (a) the first nucleoside sequence2. Two kinds of coding polypeptides, including the amino acid sequence of SEQ...
Dnmt3a2 is an immediate early gene that is identical to Dnmt3a1 except that it lacks the sequence encoding the N-terminal 219 amino acids of the enzyme, which encompasses the epitope of the antibody that was used in the current study. Intriguingly, we found increased expression of Dnmt3a2 ...
In Cyclin F, this hydrophobic patch with the amino-acid sequence MRYIL (Met-Arg-Tyr- Ile-Leu) associates with the cyclin-binding motif (Cy motif) Arginine-X-Leucine in its substrates24,25. We asked whether the interaction between Cyclin F and CDC6 is dependent on the Cyclin box domain ...
(2) upon transcription, HT–T7RNAP pulls the opposing origami arm towards itself, forcing the structure to close; (3) when the terminator sequence is reached, the T7RNAP releases the dsDNA template linker, which causes the structure to actively open to its equilibrium conformation. The T7...
(Fig.3b–d). Hence, without a proper preliminary assessment, one could falsely attribute the lack of fluorescence change to a poor (or even non-existent) affinity of RPA to these DNA sequences. In such scenarios, one could possibly change the sequence to generate a fluorescence modulation, ...