DDX21 dimerization is essential for its helicase activity and depends upon its hydrophobic LAAALA sequence.27 To generate an obligate DDX21 monomer, LAAALA amino acids 611–616 of DDX21 were mutated to RRRRRR (termed DDX21611R); an LAAALA deletion construct was also generated (termed DDX2...
Table S1. High-Confidence BioID Proximity Interactome for the Organization of RNA Biology, Related to Figures 1, 2, 3, and 4 Table S2. Correlation Analyses between Replicates or ± Conditions; Comparison of EIF4A1, G3BP1, and PABPC1 BioID Data; and Reproducibility of N- versus C-Terminally...
Gal-3 interacts with pre-mRNA at the 5′end of the introns via U1 snRNP in the nucleus where it promotes their splicing3,26. We showed by RNA-Seq analysis that Gal-3 binds to a mature mRNA after removal of introns and export in the cytosol. Our data, together with those reported by...