DDX21 dimerization is essential for its helicase activity and depends upon its hydrophobic LAAALA sequence.27 To generate an obligate DDX21 monomer, LAAALA amino acids 611–616 of DDX21 were mutated to RRRRRR (termed DDX21611R); an LAAALA deletion construct was also generated (termed DDX2...
Sequence reads were analyzed with Torrent Server 3.0 for barcode analysis and adaptor trimming. The reads were aligned with bowtie249 on refSeq mRNA downloaded from UCSC. Then we only take into account reads mapping on mRNA with an ENTREZ Gene ID using ID converter50. FISH and imaging studies...
In UBAP2LΔ and CSDE1Δ, there was dramatic reduction in G3BP1 foci assembly after arsenite treatment (Figures 7A and 7B; Table S9), a phenotype recapitulated in additional isolates (Figure S5A; a single guide RNA [sgRNA] sequence targeting a different exon was used to isolate clone UBA...
Gal-3 interacts with pre-mRNA at the 5′end of the introns via U1 snRNP in the nucleus where it promotes their splicing3,26. We showed by RNA-Seq analysis that Gal-3 binds to a mature mRNA after removal of introns and export in the cytosol. Our data, together with those reported by...