We review and discuss the biological and DNA-binding properties of the bacteriophage SPOT transcription factor 1(TF1), a DNA-binding protein belonging to the ubiquitous prokaryotic family of Type II DNA-binding proteins. We review recent information on the effects of certain mutations in TF1 on DN...
TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and theEscherichia coliintegration host factor (IHF). Distinctive to TF1, which is encoded by theBacillus subtilisbacteriophage SPO1, is its preferential binding to DNA in which thymine...
b For each TF-pair, the proportion of TF isoform-pairs that show each type of binding modality (cooperativity, TF1 or TF2 antagonized, mutual antagonism, TF1 and/or TF2 independent binding, or no binding) across DNA-baits. Names in red indicate TFs for which alternative isoforms were studied...
Tf1 insertion predominantly occurs closer to the 5′ end of genes, in regions known to have relatively open chromatin [78, 79]. These studies clearly argue for the relationships between open chromatin and preferential transposition sites. However, it should be noted that these TE insertions at ...
Recent studies have revealed that various protein factors have important roles in gene expression. These factors are involved in transcriptional regulation not only by binding to the promoter region of DNA with sequence specificity but also by interactin
However, "self-priming" from a hydrolysis product of the viral genome has been observed for the LTR retrotransposon Tf1 and most likely exists for related elements. Furthermore, in contrast to retroviruses, where DNA synthesis is initiated from the 3'-terminus of the cognate tRNA primer, ...
Effects of mutations at amino acid 61 in the arm of TF1 on its DNA-binding properties J. Mol. Biol. (1990) F. Saitoh et al. Arginine-55 in the beta-arm is essential for the activity of DNA-binding protein HU from Bacillus stearothermophilus Biosci. Biotechnol. Biochem. (1999) K.K....
Natural mitochondrial DNA (mtDNA) mutations enable the inference of clonal relationships among cells. mtDNA can be profiled along with measures of cell state, but has not yet been combined with the massively parallel approaches needed to tackle the compl
, 25.. Other members of the DNABII family include TF1 as well as the HU proteins from prokaryotes 20., 22., 23.. IHF participates in chromosome packaging, the regulation of at least 120 genes, the integration of the virus λ, the initiation of DNA replication, and the stabilizing of ...
The possibility that the several micromolar to millimolar ATP concentration might be required for saturating more than one binding site present on DnaA protein, a function specifically required for the conversion of bORC to pre-RC cannot be excluded and the mechanistic detail on these studies needs...