Helicase – The point at which the replication begins is known as the Origin of Replication. Helicase brings about the procedure of strand separation, which leads to the formation of the replication fork. It breaks the hydrogen bond between the base pairs to separate the strand. It uses e...
DNA replication, also known assemi-conservative replication, is the process by which DNA is doubled. This is an important process taking place within the dividing cell. In this article, we shall discuss thestructureof DNA, the steps involved inDNA replication(initiation, elongation and termination...
the existence of DNA polymerase can be analyzed by incorporation assay. DNA replication requires primers. We make a primer template junction (PTJ) and put it into the buffer solution of dNTPs, a reaction substrate with radioactive or fluorescent labels. Then we can detect...
Aging is associated with multiple cellular changes that are often tissue specific1. Cytosine methylation, however, stands out, as it allows for the development of pan-tissue aging clocks (multivariate age estimators) that are applicable to all human tissues2,3,4. The subsequent development of simi...
(H3K27me3)4,33,34,35. In DNMT1, the two BAH domains are both associated with the MTase domain to form an integrated structural unit15. Although previous studies indicated that these domains play a role in targeting DNMT1 to replication foci14,36, their functions remain uncharacterized to ...
We detected the formation of multiple de novo R-loops in the pachytene stage and found that these R-loops were associated with meiotic recombination during yeast meiosis. We show that transcription-replication head-on collisions could promote R-loop formation during meiotic DNA replication, and ...
The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1 cells complexed with Purα, a sequence-specific single-stranded DNA-binding protein implicated in control of gene transcription and DNA replication. These complexes can be immunoextracted from cell lysates using monoclonal ...
DNA substrates are diagramed at the bottom of each panel, and asterisks indicate 5′-32P labels. Core7 concentrations were 10, 20, 40, and 80 nM in each assay. Triangles (▵) indicate the heat-denatured substrates, and the “C” stands for control of DNA substrate alone. (B) Effects...
12. R-loops thus acting as barriers hinder DNA replication and even induce DNA breaks at common fragile sites13. RNA and DNA polymerases occurring at the same genomic loci could lead to transcription and replication collisions (TRCs) that are normally associated with constant and stabilized R-...
Al-Hashimi3 Hoogsteen (HG) base pairing involves a 180° rotation of the purine base relative to Watson– Crick (WC) base pairing within DNA duplexes, creating alternative DNA conformations that can play roles in recognition, damage induction and replication. Here, using nuclear magnetic ...