Summary Double label immunofluorescence against PCNA and BrdU clearly revealed several characteristics of DNA replication sites in synchronized CHO cells. We observed that the distribution of replication sites changed from early to late S phase, particularly in the nucleolar and perinuclear regions, and ...
Stability of the 18O-label during replication of DNA of E. coli or T2 bacteriophage conclusively eliminates the unlikely possibility that each phosphodiester bond undergoes hydrolysis with P—O cleavage and resynthesis during intracellular DNA replication. Results with E. coli suffice to eliminate such...
Our approach for studying nucleosomal interactions can greatly accelerate the understanding of fundamental mechanisms by which transcription, replication, and repair processes in a cell are modulated through DNA-histone interactions, as well as in diagnosis of diseases with abnormal patterns of DNA and ...
Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity Phosphorylation of DNA by polynucleotide kinase (PNK) takes an important role in DNA damage repair, replication and recombination. The evaluation of PNK ac... L ...
Dose-Determined Effects of HT3DR as DNA Label Upon The Liver Cell Replication Time And Pattern in the Growing RatAgonistic and affiliative interactionslion-tailed macaquesmale-male relationshipssocial toleranceSodium vanadium oxide gels, NaxV2O5路nH2O, of varying sodium content (0.12 < x < 0.32) ...
Kensuke Ogawa1, Noritada Kaji1,2, Mats Nilsson4, Taiga Ajiri5, Manabu Tokeshi2,6,Yasuhiro Horiike7 &Yoshinobu Baba1,2,8 Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological...
Notably, 15% of all UICC stage II and III CRCs are high MSI (MSI-H) showing hypermutated tumors. This results from a defective DNA mismatch repair (MMR) system, having a clear molecular origin (MLH1, MSH2, MSH6, and PMS2 inactivation), and arises from a germline or somatic mutations4...
The MCM5 antigen is a potential biomarker for cancer cell proliferation because it initiates DNA replication [6]. This antigen is related to malignancy and poor prognosis in patients with cervical cancer and inhibits the growth of cervical adenocarcinoma cells. However, the clinical significance and ...
The trained network was applied to classify the viruses from the interferometric images only, containing simultaneously SARS-CoV-2, H1N1 (influenza-A virus), HAdV (adenovirus), and ZIKV (Zika virus). Remarkably, due to the nanoscale sensitivity in the input data, the neural network was able ...
Our approach arises from the relevance of T7 DNA polymerase, which possesses multiple domains with different activities, ensures high fidelity of replication, has the property of significant conformational change, regulates geometry and activity, and fast catalysis of the chemical reaction operating at ...