Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like helicase. Here we present data, employing a comb
It is conceivable that with the advance of the replication fork, the helix of the remaining DNA will become more and more compact, which will cause greater resistance. The negative super helix leaves room for a certain buffer, which is conducive to DNA replication. On the other hand, DNA he...
DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-resolution technique with relatively easy-to-implement multi-target imaging. However, image acquisition is slow as sufficient statistical data has to be generated from spa
DNA synthesis is a complicated process involving anabolic processes to create the purine and pyrimidine nucleotide triphosphates required for replication, unwinding of the template DNA to provide access to the replication machinery, and the high fidelity process of creating complementary strands. ...
Cisco DNA Center 2.3.5.3 Rejoin task passed - dB replication happens when DR setup is ready for Manual failover. CSCwf62913 Cisco DNA Center 2.3.5.3: After system upgrade all Postgres services are in CrashLoopBackOff with no Master. CSC...
The first molecular model of DNA repeat expansions was based on strand slippage.51Looping out one or several repeats in the newly synthesizedDNA strandshould convert the loop into expansions after a second round of replication. However, this simple idea fails to explain why only a handful of all...
In the replication of DNA and the shortening of telomeres, DNA nucleotides act as magnets [2]. Magnetic beads (MB) can be used as labels to concentrate or separate biological targets from a sample, such as cells, proteins, and DNA/RNA [3]. ...
reported a noncanonical and topological flower-like DNA nanosphere, also called a DNA nanoflower, which was self-assembled from long single-stranded DNA (lsDNA) generated via rolling circle replication (RCR)/rolling circle amplification (RCA) [25]. RCR is an efficient isothermal amplification ...
Several phytoplasmas, wall-less phloem limited plant pathogenic bacteria, have been shown to contain extrachromosomal DNA (EcDNA) molecules encoding a replication associated protein (Rep) similar to that of geminiviruses, a major group of single stranded (ss) DNA plant viruses. On the basis of tha...
Full size image KILR inhibits DNA replication by sequestering the RPA1 protein To identify proteins that physically interact with KILR, we performed RNA pull-down in MCF7 cells using in vitro transcribed KILR followed by mass spectrometry. KILR-binding proteins identified by the presence of at ...