which leads to the formation of the replication fork. It breaks the hydrogen bond between the base pairs to separate the strand. It uses energy obtained fromATPHydrolysis to perform the function.
DNA replication, also known assemi-conservative replication, is the process by which DNA is doubled. This is an important process taking place within the dividing cell. In this article, we shall discuss thestructureof DNA, the steps involved inDNA replication(initiation, elongation and termination...
The double strands of DNA are separated, which requires the DNA helicase to disassemble the original double stranded DNA. Where the double chain breaks down is called a replication fork. Two replication forks can generate a replication bubble. The chemical mechanism of DNA replication is that the ...
DNAReplication 1)Semi-conservativemechanism（半保留机制）2）Replicons(复制子),origins（复制起点）,andtermini（复制终点）3）Semi-discontinuousreplication（半不连续复制）4）RNApriming（引导）Thecharacteristicofreplication:Semi-conservativeSemi-discontinuous Semi-conservativemechanism:Duringreplication,thestrandsofthe...
As the molecular properties of the replication fork are central to the regulation of replication5, we set out to investigate replication fork dynamics in totipotent cells in vivo and totipotent-like cells in culture. Results 2CLCs and totipotent embryos have a slow replication fork speed Totipotent...
Positive supercoiling is generated ahead of replication and transcription machinery as they progress along the DNA template42,43,44. Nucleosomes in chromatin limit the intertwining of the newly replicated daughter duplexes behind the polymerase during replication, driving the supercoiling to partition pri...
Single-stranded RNA virus genomes may be positive-sense (i.e., the same polarity or nucleotide sequence as the mRNA; designated as ssRNA+), negative-sense (opposite polarity; ssRNA−, needs to be transcribed for mRNA), or ambisense (a mixture of the two, with an ssRNA− replication st...
DNA replication, and cell cycle in the genes whose expression variation is associated with islands (Fig.4band Supplementary Data3), a conclusion confirmed by a Reactome analysis (Supplementary Fig.9). Noteworthy, these results were obtained when we analyzed the function of the human homologs not ...
Using the budding yeast Saccharomyces cerevisiae as a model system, we find that Dna2 — a key nuclease in DNA replication and repair — employs a bimodal interface to act with RPA both in cis and in trans. The cis-activity makes RPA a processive unit for Dna2-catalyzed ssDNA digestion, ...
By simulating DnaA kinetics, we obtained a regular DnaA oscillation coordinated with cell cycle and a converged cell size that matches replication initiation frequency to the growth rate. The relationship between the simulated cell size and growth rate, C period, D period, or initiation mass ...