Successful DNA replication is essential to maintain genome integrity and prevent disease. In human cells, over 6 billion base pairs of DNA need to be replicated accurately and packaged into chromatin each cell division cycle. Furthermore, these processes must occur in the context of defects in ...
repair. To test this possibility, we examined the expression patterns of genes in DNA repair pathways usingmicroarrays(Miura et al., 2004a). As expected, many replication and repair-related genes including APEX, RAD,MSHand XRCC are highly expressed in hESCs (X. Zeng, unpublished observations)...
^Sparks JL, et al. The CMG Helicase Bypasses DNA-Protein Cross-Links to Facilitate Their Repair. Cell. 2019 Jan 10;176(1-2):167-181.e21. ^Perry M, Ghosal G. Mechanisms and Regulation of DNA-Protein Crosslink Repair During DNA Replication by SPRTN Protease. Front Mol Biosci. 2022 Jun...
[66]. DNA mismatch repair copes with errors that arise during DNA replication, such as mismatched nucleotides or short hairpin or loop structures[67]. Base excision repair is the predominant pathway for coping with non-bulky base modifications, namely oxidative DNA base lesions, as well as AP ...
29. Cleaver, J.E., Defective repair replication of DNA in xeroderma pigmentosum. Nature, 1968. 218(5142): p. 652-6. 30. Mu, D., et al., Reconstitution of human DNA repair excision nuclease in a highly defined system. J Biol Chem, 1995. 270(6): p. 2415-8. ...
Fig. 2: Emergence of 2CLCs occurs together with or after DNA replication. a, Strategy to evaluate 2CLC emergence during the S-phase. b, After synchronization of ESCs at G1/S by double thymidine block, existing 2CLCs were removed. The remaining cells were released from the block and cultur...
et al. Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair. Mol. Cell 37, 259–272 (2010). Article CAS PubMed Google Scholar Tafel, A. A., Wu, L. & McHugh, P. J. Human HEL308 localizes to damaged replication forks and ...
DNAReplication,Recombination,andRepairGuo-MinLi(李国民),Ph.D.ProfessorJamesGardnerEndowedChairinCancerResearchUniversityofKentuckyCollegeofMedicineSelfintroduction•DNArepair,genomeinstability,andcancer•TrinucleotiderepeatinstabilityandneurodegenerativediseasdesResearchInterest•HypermutabilityandmismatchrepairdeficiencyinRER...
general, the accuracy of replication is extremely high. However, there do occur replication errors. It is estimated that approximately one error is introduced per billion base pairs during each cycle of replication. The cells do possess the capability to repair damages done to DNA to a large ...
DNA mismatch repair(MMR) is a highly conservedDNA repairsystem, which is essential to all organisms. It has an important role in maintaininggenomic instability.MMRtargets mismatches that arise during replication andhomologous recombination(HR), as well as repairing mismatches that occur in DNA followin...