DNA damagecrosslink repaircancermismatch repairexcision repairThe replisome quickly and accurately copies billions of DNA bases each cell division cycle. However, it can make errors, especially when the template DNA is damaged. In these cases, replication-coupled repair mechanisms remove the mistake or...
DNA2 and EXO1 deficiency leads to ICL sensitivity but does not increase ICL sensitivity in the absence of FANCD2. This is the first demonstration of the redundancy of human resection nucleases in the HDR step in replication-coupled repair, and suggests that DNA2 may represent a new mediator ...
DNA-protein crosslinks (DPCs) are caused by environmental, endogenous, and chemotherapeutic agents and pose a severe threat to genome stability. We use Xenopus egg extracts to recapitulate DPC repair in vitro and show that this process is coupled to DNA replication. A DPC on the leading strand ...
Our analysis of synchronized cells and asynchronously proliferating live single cells revealed a consistent order of replication-coupled destruction during both S-phase entry and DNA repair; Cdt1 is destroyed first, whereas p21 destruction is always substantially later than that of Cdt1. These ...
Role of Dnl4–Lif1 in nonhomologous end-joining repair complex assembly and suppression of homologous recombination Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) in bacteria and eukaryotes. In Saccharomyces cerevisiae, there are pairwise phy... YuZhanAsracNonhomooousnd...
Concomitant chromatin assembly and DNA duplication is essential for cell survival and genome integrity, and requires newly synthesized histones. Although the N-terminal domains of newly synthesized H3 and H4 present critical functions, their requirement for replication-coupled chromatin assembly is controvers...
which reconciles the discrepancy of different signatures for origin efficiency. Lastly, our work also provides new insights into how 3D genome structural dynamics, particularly the intra-TAD physical structures, may regulate other nuclear processes on chromatin templates such as DNA repair, adding a ne...
Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system ...
Cellular and molecular conse- quences of defective Fanconi anemia proteins in replication-coupled DNA repair: mechanistic insights. Mutat. Res. 668:54-72.Thompson LH, Hinz JM (2009) Cellular and molecular consequences of defective Fanconi anemia proteins in replication-coupled DNA repair: mechanistic...
CAF-1 promotes replication-coupled DNA repair independently of the error-prone and error-free branch of PRR.Violena PietrobonKarine FréonJulien HardyAudrey CostesIsmail IraquiFrançoise OchsenbeinSarah A.E. Lambert