Whether we used cells in tissue culture or specific tissues we found that the best quality of DNA was always obtained from isolated nuclei. However, it is also possible to extract large size DNA directly from frozen tissues, cells in tissue culture or protoplasts. Such techniques will be ...
DNA was isolated from cultured mammalian cells, liver tissue and Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young ...
The epithelial tissues present on the surface of the feces were used as source for isolation of genomic DNA. The DNA isolated from fecal tissue was found to be similar as those obtained from other body tissues such as skin, brain, liver, kidney, and muscle. The quality of DNA was checked...
2.5 mg mouse liver: 600–3,000 ng20–35 µg A260/2801.7–1.91.7–1.9 Elution volume5–30 µl60–100 µl Preparation time~40 min/prep (excl. lysis)~20 min/prep (excl. lysis) Applications NucleoSpin Tissue XS DNA isolation from tissue (e.g., mouse kidney, laser micro-dis...
Cell-free DNA (cfDNA) derived from tumours is present in the plasma of cancer patients. The majority of currently available studies on the use of this circulating tumour DNA (ctDNA) deal with the detection of mutations. The analysis of cfDNA is often dis
USA). Samples that produced no PCR products after 40 cycles were considered undetectable and the Ct number set to 40 for statistical purposes. Mitochondria were isolated from whole liver tissue of wild-type male C57BL/6 mice using Mitochondria Isolation Kit for Tissue (Thermo Fisher Scientific, ...
aberrant end motifs with those of other cancer types, the researchers observed that the profile of plasma DNA end motifs originating from the same organ, such as the liver, placenta, and hematopoietic cells, generally clustered together, indicating that such markers carry tissue-of-origin ...
We have fractionated oviduct tissue extracts by using a combination of ion-exchange and DNA-Sephadex chromatography. By comparing the electrophoretic patterns of proteins eluted from competing specific and nonspecific DNA columns, we isolated a fraction which bound with specificity to columns containing th...
For nuclei isolation, cells were incubated for 15 min at 4 °C in buffer I (10 mM Hepes pH 8, 1.5 mM MgCl2, 10 mM KCl), lysed by adding 1% NP-40, and shaken using a vortex for 30 s. Nuclei were pelleted by centrifugation (10 min at 1600 × g) and washe...
DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet is ...