Rinsing an animal is inconsistent at removing exogenous DNA contaminants. Rinse water is a poor negative control for exogenous DNA contamination. A swab of the animal exterior performs as a better negative control. Disposable syringes are effective for obtaining gut content for DNA extraction.关键词:...
Liver and kidney chromatin and DNA were fractionated in acrylamide columns with immobilized readily extracted non-histone chromosomal proteins (RE-NHCP). C... K Ep,S Ki,A Ip - 《Biochemistry》 被引量: 0发表: 1977年 Application of solid phase extraction disk in the determination of acrylamide ...
DNA can beextracted from many types of cells. Thefirst stepis to lyse or break open the cell. This can be done by grinding a piece of tissue in a blender. After the cells have broken open, a salt solution such asNaCl and a detergent solutioncontaining the compoundSDS (sodiumdodecyl sulf...
which is observed in approximately 50% of EOC and results in DNA repair deficiencies through homologous recombination [121]. Several studies have indicated that patients withBRCA-mutant HGSOC tend to exhibit a higher likelihood of survival compared to those...
It remains to be seen, however, which of the interpretations would be supported with future versions of the DNAm clock, or, if our findings are specific to the source of the signal derived from human physical activity time series properties, known to be associated with both of the diseases....
ficientextractionofDNA,RNA,andproteinfromthesametissueblock. However,thefewcommerciallyavailablesimultaneousextractionkitshavenotbeenevaluated.Wecom- paretheTriplePrep(GEHealthcare)andAllPrep(Qiagen)kitsusinggood,intermediate,andpoorquality tissuewithspecialistsingle-extractmethods:Puregene(DNA),RNeasy(RNA),and...
DNA damage caused by IR or UV. In each of the two treatments, the data consisted of four biological states, i.e. sham-treated, 2 h-, 6 h-, and 24 h post UV- or IR-treatment. A gene expression profile consists of eight inter-groups, corresponding to four states from the two ...
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