DNA replication inhibitor of various exogenous carcinogenic stimuli, including carcinogens such various of potential carcinogens such as carcinogens, or a test or diagnostic method of disease activity is involved or the expression of Frag1 screening methods and of anti-cancer agents, as well as a kit...
Biochem Biophys Methods, 2005, 63(1): 53-68. (编辑 曹大刚) - 5 - Comet assay,a new method for detecting DNA damage QU Ying1, QI Hao2, LUO Ming-zhi2,3 , GUO wei2, LIU Miao2 (1. Institute of Photonics Photo-Technology, Northwest University, Xi’an 710069, China;2. College of...
The comet-FISH technique: a tool for detection of specific DNA damage and repair. Methods Mol. Biol. 291, 107–119 (2005). CAS PubMed Google Scholar Speit, G. & Hartmann, A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage. Methods Mol. Biol. 291, 85...
j, Fluorescence polarization dose–response curves using 10 nM Cy5-labelled 15-bp oligonucleotide with a single THF damage site (Cy5–15-bp-DNATHF) mixed with UV-DDB (0.3–200 nM). The interaction is measured and plotted as described in Methods. All data are shown as mean ± s.d. of ...
Methods for detection of DNA damage and repair can be applied to identify, visualize and quantify the DNA damage formation and repair events, and they enable us to illustrate the molecular mechanisms of DNA damage formation, DNA repair pathways, mutagenesis and carcinogenesis. Ever since the ...
In this Review, we introduce the biochemistry of cytosine and thymine modifications and discuss established detection methods, such as bisulfite sequencing and its modifications, as well as newer methods, which have been developed to overcome the substantial obstacles associated with studying these ...
NUCLEIC ACIDS | Electrochemical Methods E.Paleček, inEncyclopedia of Analytical Science (Second Edition), 2005 DNA Damage DNAin living organisms is a critical target of various agents, includingoxidative metabolism, photoexcited dyes, radiation, and other agents that induce damage of bases and/or ...
Methods for quantifying DNA damage, as well as repair of that damage, in a high-throughput format are lacking. Single cell gel electrophoresis (SCGE; comet assay) is a widely-used method due to its technical simplicity and sensitivity, but the standard comet assay has limitations in reproducibil...
(cometassay)、或碱性洗脱测定、或滤器洗脱测定)检测提取材料中的dna端部(kohnkurtw.、leonardc.erickson、reginaa.g.ewig和charlesa.friedman.fractionationofdnafrommammaliancellsbyalkalineelution.biochemistry,1976,15(21),第4629-4637页;dnadamagedetectioninsitu,exvivo,andinvivo(2011).methodsandprotocols.springer...
Methods Mol Biol, 2018, 1699: 3-21. [5] Turgeon MO, Perry Nicholas JS, George P, et al. DNA Damage, Repair, and Cancer Metabolism[J]. Front in Oncol, 2018, 8(6): 15-20. [6] Shubassi G, Robert ...