To reveal the role of each DNA-binding domain of p53 in these processes, we investigated two p53 mutants lacking either of two DNA-binding domains; structured core and disordered C-terminal domains, using sub-millisecond single-molecule fluorescence microscopy. We found that the C-terminal domain...
a我们应将主要以力集中在周围 We should mainly concentrate by the strength in periphery[translate] acollection of monomeric units, and bond-forming processes[translate] aligand to the DNA-binding domain of the p53 transcription factor.[translate]...
Stabilising the DNA-binding domain of p53 by rational design of its hydrophobic core. Protein Eng. Des. Sel. 22 (7), 421-430.Khoo KH, Joerger AC, Freund SM, Fersht AR (2009) Stabilising the DNA-binding domain of p53 by rational design of its hydrophobic core. Protein Eng Des Sel 22...
aforeign clrade 外国clrade[translate] aI won't let tomorrow Slip away 我明天不会让滑倒[translate] aon fighting for 在战斗为[translate] aligand to the DNA-binding domain of the p53 transcription factor ligand到p53副本因素的脱氧核糖核酸束缚的领域[translate]...
Defining the p53 DNA-binding domain/Bcl-XL-binding interface using NMR. FEBS Lett. 559 : 171-174.Petros A. M., Gunasekera A., Xu N., Olejniczak E. T. & Fesik S. W. Defining the p53 DNA-binding domain/Bcl-x(L)-binding interface using NMR . FEBS Lett 559 , 171–174 (2004)....
The DNA-binding domain of p53 (p53DBD) interacts with defined DNA sites and is the main target for mutations in human primary tumors. Here, we show that the CWWG motif, found in the center of each consensus p53 half-site, is a key player in p53/DNA interactions. Gel-mobility-shift ...
TP53(tumor protein p53)作为肿瘤抑制基因主要由11个外显子及10个内含子构成,其编码的p53蛋白由393个氨基酸残基组成,包含了五大功能结构域:N端反式激活结构域(transcription activation domain,TAD)、富含脯氨酸结构域(proline-rich domain,PRD)、核心DNA结合结构域(DNA-binding domain,DBD,p53C)、四聚化结构域(tetr...
Binding to a p53 site could be observed even when the digestion mixture contained no detect- able intact p53 and consisted predominantly of the core domain. The shifted DNA band had a mobility faster than that of the intact p53-DNA complex, suggesting that a proteolytic fragment of p53, ...
Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influe...
The code structure of the p53 DNA-binding domain and the prognosis of breast cancer patients.Motivation: The tumor-suppressor gene TP53 mutations are diverse in the central region encoding the DNA-binding domain. It has not been clear whether the prognostic significance for survival in breast ...