Using high-throughput chromatin conformation capture, we generated chromatin interaction maps forandat a high resolution and characterized the conservation and divergence of chromatin organization in these two species. Large-scale chromatin structures, including A/B compartments and topologically associating ...
Single-molecule sequencing and chromatin conformation capture enable de novo reference assembly of the domestic goat genome. Nat Genet. 2017;49:643. Daccord N, Celton J-M, Linsmith G, Becker C, Choisne N, Schijlen E, et al. High-quality de novo assembly of the apple genome and ...
Here, we report a high-quality, chromosome-scale reference genome of ginger ‘Zhugen’, a traditionally cultivated ginger in Southwest China used as a fresh vegetable, assembled from PacBio long reads, Illumina short reads, and high-throughput chromosome conformation capture (Hi-C) reads. The ...
At first glance, the short- and long-range intrachromosomal contacts that form chromatin loops and CTs would seem to discourage interchromosomal interactions. However, techniques ranging from traditional genetics to fluorescent in situ hybridization (FISH) and chromosome conformation capture (e.g. 3C,...
Furthermore, epigenetic and chromosome conformation capture data are consistent with rs7090445 localizing within a chromatin contact domain and overlapping a B-cell enhancer. This interval, anchored by CTCF binding sites, forms a 'loop domain' which is expected to bring two regions of RUNX3 binding...
(Supplementary Table1). We constructed high-throughput chromosome conformation capture (Hi-C) library to anchor scaffolds to chromosomes. Totally 54.7 Gb uniquely mapped valid Hi-C reads were used for scaffolding by LACHESIS software15. As a result, 1.203 Gb (97.5%) of the assembly were...
Approximately 2 g of fresh leaves collected from the same PI464715 accession was used for Hi-C sequencing. Hi-C libraries were constructed following Miele et al.52with chromatin extraction; digestion; and DNA ligation, purification and fragmentation. Hi-C sequencing was performed using the Illumi...