High-throughput genome-wide chromatin conformation capture assay (Hi-C) is routinely used to profile long-range genomic interactions and three-dimensional organization of genomes. A key application of Hi-C is the comparative analysis of genomic interactions across different time points, cellular ...
To identify cell-type-specific chromatin loops on a genome-wide scale, we generated 3D chromatin interaction maps at single-cohesin peak resolution (about 2-kb) using a modified ChIA-PET assay (Extended Data Fig.1a,Methods). In brief, this chromatin conformation capture assay incorporates an imm...
Micro Capture-C (MCC) is a chromatin conformation capture (3C) method for visualizing reproducible three-dimensional contacts of specified regions of the genome at base pair resolution. These methods are an established family of techniques that use proximity ligation to assay the topology of chromatin...
最近做了chromatin conformation capture,感觉很困难啊,酶切好像效率很低,做完后的文库也扩不出来,不知道什么问题。做过的虫子来说说? 下图中,U是没有酶切的,D1、D2是分别加了1、2次内切酶的(400U每次),L是连接后的产物。好像没什么区别? N0 14 3C 酶切酶连 验证 2015-05-14 09 时 29 分副本.jpg...
Several recently developed experimental methods, each an extension of the chromatin conformation capture (3C) assay, have enabled the genome-wide profiling... Ferhat,Ay,Thanh,... - 《Bmc Genomics》 被引量: 20发表: 2015年 Exploring 3D chromatin contacts in gene regulation: The evolution of appro...
Advances in methodologies based on chromatin conformation capture (3C) have shed light on the genome-wide organization of chromatin in developmental processes. Here, we review recent discoveries regarding the regulation of three-dimensional (3D) chromatin conformation, including promoter-enhancer looping, ...
Bickhart, D., Rosen, B., Koren, S.et al.Single-molecule sequencing and chromatin conformation capture enablede novoreference assembly of the domestic goat genome.Nat Genet49, 643–650 (2017). https://doi.org/10.1038/ng.3802 Download citation ...
A key advance in our understanding of gene regulation came with the finding that the genome undergoes three-dimensional nuclear folding in a genetically determined process. This 3D conformation directly influences the association between enhancers and their target promoters. This complex interplay has ...
We developed 4C technology (chromosome conformation capture (3C)-on-chip), which allows for an unbiased genome-wide search for DNA loci that contact a given locus in the nuclear space. We demonstrate here that active and inactive genes are engaged in many long-range intrachromosomal interactions...
How chromosomes fold and how distal genomic elements interact with one another at a genomic scale have been actively pursued in the past decade following the seminal work describing the Chromosome Conformation Capture (3C) assay. Essentially, 3C-based technologies produce two-dimensional (2D) contact...