Expanding the list of sequence-agnostic enzymes for chromatin conformation capture assays with S1 nucleaseENZYMESGENOMESCHROMATINThis study presents a novel approach for mapping global chromatin interactions using S1 nuclease, a sequence-agnostic enzyme. We develop and outline a pr...
Micro Capture-C (MCC) is a chromatin conformation capture (3C) method for visualizing reproducible three-dimensional contacts of specified regions of the genome at base pair resolution. These methods are an established family of techniques that use proximity ligation to assay the topology of chromatin...
et al. Hi-C: a comprehensive technique to capture the conformation of genomes. Methods 58, 268–276 (2012). Article CAS PubMed Google Scholar Mumbach, M. R. et al. HiChIP: efficient and sensitive analysis of protein-directed genome architecture. Nat. Methods 13, 919–922 (2016). ...
Hi-C is an adaptation of the chromosome conformation capture (3C) methodology20 that identifies long-range chromosome interactions in an unbiased fashion without a priori target site selection. The frequency of long-range consensus interactions decays rapidly as linear distance along a chromosome ...
Formation is driven by protein-chromatin interactions mediated by RNA that could partition the genome into functionally open or closed domains, observed by chromosome conformation capture techniques, providing an evolutionary constraint for maintaining gene-rich domains together (Ghanbarian and Hurst, 2015)...
The advent of chromosome conformation capture (3C) and related methods allows examination of pairwise chromatin interactions at the molecular level (∼1–100 nm) in cell populations (Gibcus and Dekker, 2013). 3C-based methods can delineate long-range chromatin looping interactions and have been ...
Chromosome conformation capture-based methods Chromatin Epigenomics Epigenetics Histone analysis Background As a technique for probing genome-wide chromatin accessibility in a small number of cells in vivo, the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) has been ...
These are evidenced by the capture of Dpn I fragments with internal (non-methylated) GATC sites (Additional File 2, Figure S2 and Table S1). These internal sites would have been protected from DAM methylation for an extended period while DAM was expressed in the relevant cells. We observed ...
Faculty Opinions recommendation of Studying physical chromatin interactions in plants using Chromosome Conformation Capture (3C). 来自 Semantic Scholar 喜欢 0 阅读量: 6 作者:X Deng,YM Kong DOI: 10.3410/f.1164404.626149 年份: 2009 收藏 引用 批量引用 报错 分享 ...
Chromosome conformation capture (3 C) assays 3C assays coupled with PCR were performed as described previously [20]. Briefly, HCC cells were cross-linked with 2% formaldehyde, and the fixation was stopped using glycine. Nuclei were collected, suspended in 1× restriction enzyme buffer in the pres...