Three different methods, PCR, seminested-PCR, and PCR-RFLP, were applied in detecting PNSSP. The prevalence of PNSSP was 73路9%, the penicillin-resistance rate of children's group and adults' group were 87路8% and 31路2%, respectively. The PCR method had difficulty in identifying ...
Four DNA extraction protocols using guanidine isothiocyanate (GIT), TRIzol, phenol: chloroform: isoamyl alcohol (PCI) and chelexR 100 resin were compared for use in nested polymerase chain reaction (nPCR) based detection of GaHV-2, responsible for causing Marek's disease in poultry. Atotal of...
To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV)-testing specimen, the laboratory diagnostic accuracy of PCR on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EayMAG, bioM茅rieux vs Qiagen)...
2. Materials and Methods 2.1. Cloning of SbERD4 Gene and In Silico Analysis The SbERD4 gene was made full length through the rapid amplification of the cDNA end (RACE) technique using an EST (Sal C38; GenBank no. EC906161) clone and gene-specific primers (Table S1). The gene was ...
After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) shall be disinfected. Therefore, the selection of an effective disinfectant plays an important role. With the availability of different methods for virus quantification, we...
Methods We investigated the biodistribution of SARS-CoV-2 among different tissues of inpatients with coronavirus disease 2019 (COVID-19) diagnosed based on symptoms and radiology and confirmed by SARS-CoV-2 detection. This study was approved by the ethics commissions of the participating hospitals, ...
Ovary development is an important determinant of the procreative capacity of female animals. Here, we performed genome-wide sequencing of long non-coding RNAs (lncRNAs) and mRNAs on ovaries of 1, 3 and 8 months old Hu sheep to assess their expressio
(dPCR) provide sensitive and specific detection, and precise quantification of nucleic acids. Both technologies have similarities, but they have differences that make one or the other the more adapted choice for specific applications. Find out how qPCR and dPCR compare for d...
Therefore, in vivo studies are currently underway to determine the effects of LAB-fermented proteins in the presence of intestinal and skeletal interactions. Materials and methods Materials Dairy- and plant-derived protein powder samples were purchased from commercial sources. The bacterial strains Lacti...
Assessment of the Genetic Diversity among Arcobacters Isolated from Poultry Products by Using Two PCR-Based Typing Methods In this study, enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were optimized for char... K Houf,L De Zu...