PCR techniques are recommended to be used for identification of Brucella instead of isolation, which is dangerous and complex.doi:10.12691/ajmr-7-1-2M. O. ElhajA. T. AbdelnassirE. EnaamScience and Education Publishing Co. LtdAmerican Journal of Microbiological Research...
SummaryDifferent PCR-based DNA fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the Acinetobacter calcoaceticus-A. bau... J Vila,MA Marcos,Jimenez de Anta, M. T. - 《J.med.microbiol》 被引量: 268发表: 1996年 Molecular genetic techniques for typing of Bartone...
antigen stimulation than in the negative cases (p < 0.0001). Detection rates of PCR techniques and flow Cytometry exceeded that of bacterial isolation (p < 0.0001) and exhibited a strong correlation. Conclusions The skin test suffers from interference from non-tuberculous mycobacteria able ...
We compared different sampling and culture techniques for the detection of GBS. Methods A total of 300 swabs was taken from 100 pregnant women at 35-37 weeks of gestation. For each subject, one rectovaginal, one vaginal and one rectal ESwab were collected. Plating onto Columbia CNA agar (...
This feature makes its utilization as molecular marker very convenient; the DNA allele polymorphism can be observed by using low-cost laboratory techniques like Agrose gel or PAGE gel electrophoresis, unlike RFLP markers that require restriction enzyme digestion and SNP markers that require florescent ...
Polymerase chain reaction (PCR) Immunochemistry (ICC) Mammalian cell culture Clinical analysers Trace Analysis How does Ultrapure Water Help Chromatography? The improved sensitivity of widely used advanced chromatography techniques, such as LC-MS and High-Performance Liquid Chromatography (HPLC), demand...
As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production ...
2C). The H15 isolate was molecularly identified via extraction of the genomic DNA and amplification of rRNA by PCR. Using 16S rRNA sequence data, the phylogenetic analysis of the bacterial strain H15 suggested that this strain is closely related to K. pneumoniae and it was deposited in the ...
The aim of the present study is thus to investigate the biological role of hypoxia-induced ANGPTL4 in SGC progression. We determined that ANGPTL4 expression was specifically induced by hypoxia in SGC cell lines, and we used siRNA knockdown (KD) techniques to evaluate the role of ANGPTL4 in ...
Different sequences were fused at the 5′ end of GFP using XmaI/SpeI restriction sites (Table S1). For all experiments with endogenous Bienertia sequences, DNA fragments were amplified by PCR from cDNA, generated from Bienertia mRNA isolated by the GeneJET Plant RNA Purification Kit (Thermo ...