气荚膜梭状芽孢杆菌A型PCR试剂盒ClostridiumperfringenstypesA产适用于各种 RNA制品的反转录反应以及随后的PCR扩增。它采用M-MLV进行反转录反应,能够获得较长的反转录产物。同时,在20ul反转录体系和50ul PCR反应体系中,还可以一次性得到足够量的PCR产物用于后续的克隆实验。本试剂盒中配制的酶均为进口的酶。RT酶采用进...
The present invention refers to a method for detecting and typifying 20 types of HPV, mainly to a method by polymerase chain reaction (PCR) in multiplex format. The novel design for diagnosing HPV by multiplex PCR, allows, in a reduced number of reactions, to identify the presence and the...
The molecular characterization of the ESBL was based on classical PCR and sequencing using specific primers of the CTX-M, SHV and TEM genes. CTX-M, SHV and TEM genes were searched in DNAs extracted from 30 isolates positives to ESBL production. CTX-M-15 was found in the 30 isolates, ...
Torque teno sus viruses (TTSuV) were detected in the pig population in the Czech Republic by a nested PCR technique. The prevalence of individual TTSuV was found to be 42.9% (TTSuV1) and 46.7% (TTSuV2). The prevalence for TTSuV 1 and TTSuV2 was determ...
viral meningitis and enterovirus cases were subjected to amplification of partial VPI gene by RT-PCR.Results:Sequencing and phylogeneiic analysis of the partial sequences identified presence of human echovinis and human coxsackie viruses.It was found that echovinis 11 was the commonly circulating ...
多重RT-PCR同时检测鸭病毒性肝炎RNA病毒DVH2型Dear Editor,Duck virus hepatitis(DVH)is caused by at least threedifferent RNA viruses,including duck hepatitis A virus(DHAV),duck astrovirus type 1(DAstV-1),and duckastrovirus type 2(DAstV-2).The first of these,DHAV,has been classified into ...
For adaptation of FMD virus (FMDV), BHK-21 cell line was used. BHK-21 cell subculture was done from preserved cultured bottle of Foot and Mouth Disease (FMD) Research Laboratory of Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka. RT-PCR confirmed selected three different positive...
The efficacy of the human papillomavirus type 16 (HPV-16)/HPV-18 AS04-adjuvanted vaccine against cervical infections with HPV in thePapillomaTrial againstCancerin YoungAdults (PATRICIA) was evaluated using a combination of the broad-spectrum L1-based SPF10PCR-DNA enzyme immunoassay (DEIA)/line ...
BiotypeLarvaeNERICAOrseolia spPCRThe study compares the impact due to rice stem borers in two sites (Yaounde and Ntui). It also shows the diversity of the African Rice Gall Midge (AfRGM) biotypes in southern Cameroon (Santchou, Ndop, Tonga, Ebolowa, Baigom, Yaounde and Ntui). The New...
The expression of bax and bcl2 genes was performed with Real-time-PCR technique and data analysis was performed by one-way analysis of variance. Results : Bcl2 and Bax gene expression in both supplemental aerobic and supplemental interval groups were significantly different from the control group ...