In this review, we introduce the history of technology development, principle, and instrument platform types of digital PCR in detail. Then, we summarize the application of this technology in GMO quantification, disease diagnosis, environment and food supervision. Finally, we describe the application ...
The results of PCR–RFLP completely coincided with those of sequence analysis, and thus PCR–RFLP is a useful technique for determining the ITS1 type in Fasciola species. 展开 关键词: characterization DNA infections molecular genetics nucleotide sequences parasites parasitology parasitoses polymorphism ...
Antimicrobial Susceptibility Pattern of Clostridium difficile and Its Relation to PCR Ribotypes in a Swedish University Hospital All 238 Clostridium difficile isolates were susceptible to metronidazole and vancomycin, whereas 84% and 1% were resistant to clindamycin and fusidic acid... O Aspevall,A Lun...
As part of the ongoing struggle between phages and the bacterial immune system, the CRISPR system has evolved into various types, each with distinct functionalities2. Type II Cas9 is the most extensively studied of these systems and has diverse subtypes. It remains uncertain whether members of ...
2 PCR/RT-PCR amplification results of three types of tea plant viruses TPNRBV)以及茶树上已报道的 TPLPV,GLRaV-7, 采用 PCR,RT-PCR 对采集的 1 869 份样品进行检测. 结果表明,利用 OTaGV,CCV1,TPNRBV 特异性引 物能够扩增到与预期大小相近的 738,707 和 315 bp 目的片段(图 3),而 TPLPV,GLR...
Development and validation of cost-effective SYBR Green-based RT-qPCR and its evaluation in a sample pooling strategy for detecting SARS-CoV-2 infection in the Indonesian setting Article Open access 20 January 2024 A new SYBR Green real-time PCR to detect SARS-CoV-2 Article Open access 26...
Molecular and chemical characterization of the lipopolysaccharide O‐antigen and its role in the virulence of Yersinia enterocolitica serotype O:8 The Y. enterocolitica O:8 (YeO8) O-antigen repeat units consist of five sugar residues: N-acetyl- d -galactosamine (GalNAc), d -galactose (Gal), ...
Identification of a novel chimeric gene, orf725 , and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion ... A novel chimeric gene with a 5′ end containing the nearly complete sequence of the coxI gene and a 3′ end showing homology with ...
The aim of the work described in this thesis is the development of microarray technology using new methods and tools, on the one hand and, on the other, its employment to address basic scientific questions. The starting point is the deve... Μαργαρίτης, Αθανάσιο...
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, bu...