Studies of the yeast protein farnesyltransferase (FTase) have shown that the enzyme preferentially farnesylates proteins ending in CAAX (C = cysteine, A = aliphatic residue, X = cysteine, serine, methionine, alanine) and to a lesser degree CAAL. Furthermore, like the type I protein ...
Taken together, it seems more likely that the substitution of Tyr by His at residue 68 in the first transmembrane leads to 26RFa-mediated intracellular Ca2+ mobilization through Gαq/PLC/IP3 and L-type Ca2+ channel. In conclusion, as shown in Fig. 10, we provide evidence that the ...
RMSD reflects predicted structural deviation from the initial structure, whereas RMSF (Root Mean Square Fluctuation) displays the average predicted fluctuation of each residue over the full course of the 500-ns simulation, here normalized to the WT structure (Fig. 2B). The RMSF prediction for α-...
5a,b). These gRNAs installed splice variants at residue E1091 of EGFR in the regulatory C-terminal domain. Using cell viability assays, we confirmed that these splice variants increased sensitivity to the EGFR inhibitors gefitinib and osimertinib (Fig. 6a), as well as other chemically distinct ...
(N.B., the 1-letter abbreviation of the amino acid is followed by ARS1 where “1” indicates the cytoplasmic form), RARS1, and MARS1, but not any other MSC constituent (Fig.4f, g). Binding was limited to wild-type SPEAR, not to the 2-nt GU-mutant. Two non-MSC synthetases, i...
It is located close to residue 670 in the tentative amino acid sequence of the entire polypeptide. Trypsin has a highly limited action on the native hexon protein. Only three peptide bonds are fully susceptible to cleavage. These were found to occur in the N-terminal third of the protein, ...
Amino Acid Patterns Within Short Consensus Repeats Define Conserved Duplicons Shared by Genes of the RCA Complex Complement control proteins (CCPs) contain repeated protein domains, short consensus repeats (SCRs), which must be relevant to diverse functions such as co... CA Mclure,RL Dawkins,JF ...
One of the first solutions to this problem was developed by (Baldi et al., 2000), who started from amino acid sequence alone and trained feedforward neural networks as binary classifiers on residue...Kamat,A.P. and Lesk,A.M. (2007) Contact patterns between helices and st...
Sequence Homology, Amino AcidSubstrate SpecificityWe describe the sequence changes of a number of mutations of the Aspergillus nidulans xanthine dehydrogenase (XDH). We have located the amino acids affected by these changes in the three-dimensional (3D) structure of aldehyde oxido-reductase (MOP) ...
Over the region of homology, there is ∼47% identity between flotillin and ESA at the amino acid level. Of the potential phosphorylation sites in flotillin, only the protein kinase C phosphorylation site at residue 150 and the tyrosine phosphorylation site at residue 152 are conserved in ESA....