Ning, K., Chong, K.F., Leong, H.W.: De Novo Peptide Sequencing for Mass Spectra Based on Multi-Charge Strong Tags. The Fifth Asia-Pacific Bioinformatics Conference (APBC2007) (2007) 287-296Ning K, Chong KF, Leong HW, De novo peptide sequencing for mass spectra based on multi charge...
De novo peptide sequencingHybrid peptide sequencingMS validationMS evidenceFunctional annotationHigh resolution mass spectrometry has revolutionized proteomics over the past decade, resulting in tremendous amounts of data in the form of mass spectra, being generated in a relatively short span of time. ...
de novo peptide sequencing algorithms的原理De novo peptide sequencing 指的是从原始蛋白质混合物中从头测序肽段的过程。这个过程主要包括以下几个步骤: 1. 蛋白质消化:首先,将蛋白质样品酶解成小肽段。这一步通常使用蛋白酶(如胰蛋白酶、胃蛋白酶等)来将蛋白质分解成肽段。 2. 肽段分离:消化后的混合物中...
The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides wit
一、概述 由于难以获得100%的蛋白氨基酸序列覆盖率,蛋白组de novo测序成为了蛋白测序的难点,由Ac-LysargiNase(N端蛋白酶)和胰蛋白酶构成的镜像酶组合可以解决这个问题并具有稳定性,这2种消化位点互补的酶能够产生目标蛋白的镜像b,y离子,基于镜像原理设计的算法pNovoM
De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid ...
We present DeepNovo-DIA, a de novo peptide-sequencing method for data-independent acquisition (DIA) mass spectrometry data. We use neural networks to capture precursor and fragment ions across m/z, retention-time, and intensity dimensions. They are then further integrated with peptide sequence patte...
网络多肽从头测序;测序 网络释义
Interpretation of these CID spectra was based on rules established for the fragmentation of the synthetic peptides. This study shows that derivatization with ansa may be useful in the de novo sequencing of peptides.Previous article in issue Next article in issue With the advent of “soft ...
The identity of the peptides was confirmed by time of flight matrix-assisted laser desorption ionization mass spectrometry (University of Alabama at Birmingham Core Facility). Inhibition of the CaM·Stains-all Complex CaM was suspended in water (300 μm final concentration) and aliquoted. All the...