此外,在测序过程中可能会发生拼接错误等问题,为了确保测序结果的准确性,在测序之前对样品进行充分的准备,以及正确处理样品都是很有必要的。 Fig. 1 Denovo测序流程图(图片参考:基于质谱和生物信息学分析的小菜蛾蛋白质鉴定 - 中国知网 (hzau.edu.cn),Highly Robust Denovo Full-Length Protein Sequencing | Analyti...
最后,牟合蛋白科技有限公司团队首发的蛋白质De novo sequencing,即蛋白质从头测序(也称全新蛋白测序),其不依赖任何蛋白质数据库,不遗漏可能肽段、点突变及未知修饰的特点突破了传统质谱测序的局限,测序覆盖率高,可与AlphaFold完美结合,对未知蛋白作出结构预测,打开蛋白质结构预测的新世界。 参考文献: [1]Callaway E....
With the increasing accessibility of Fourier transform (FT) mass spectrometers, top-down/middle-down MS/MS characterization of protein sequences is rapidly gaining popularity. Compared to conventional bottom-up sequencing, the top-down/middle-down approach offers the advantages of fast sample ...
De novo protein sequencing and function determination by mass spectrometryLoo, J ADu, PKilby, G WMueller, W TStevenson, T IWhitton, MLoo, R R O
Fast: Sequencing can be accomplished within 10 working days. Additional 2~3 weeks would be needed if the discrimination of leucine & isoleucine is required. Sensitive: Full coverage can be obtained from 50-100 μg of protein. Robust: We guarantee 100% coverage for the VJ and VDJ region. ...
We present DeepNovo-DIA, a de novo peptide-sequencing method for data-independent acquisition (DIA) mass spectrometry data. We use neural networks to capture precursor and fragment ions across m/z, retention-time, and intensity dimensions. They are then further integrated with peptide sequence patte...
Here we present GlycanFinder, a database search and de novo sequencing tool for the analysis of intact glycopeptides from mass spectrometry data. GlycanFinder integrates peptide-based and glycan-based search strategies to address the challenge of complex
本研究采用首次采用de novo转录组组装方法组装了产黑色素短梗霉转录组,并通过转录组对产黑色素短梗霉PMLA生物合成机理进行了探究,本研究结果为进一步研究和利用产黑色素短梗霉菌种提供思路。 第一作者 王舸楠:博士研究生,专业为轻工...
本研究采用首次采用de novo转录组组装方法组装了产黑色素短梗霉转录组,并通过转录组对产黑色素短梗霉PMLA生物合成机理进行了探究,本研究结果为进一步研究和利用产黑色素短梗霉菌种提供思路。 第一作者 王舸楠:博士研究生,专业为轻工技术与工程,主要研究内容为基因组、转录组组装、注释及分析;生物信息;机器学习;丝状...
Large-scale exome sequencing identified 18 novel genes for neuroticism in 394,005 UK-based individuals Xin-Rui Wu Ze-Yu Li Jin-Tai Yu Nature Human Behaviour(2024) Nature Genetics (Nat Genet)ISSN1546-1718(online)ISSN1061-4036(print)