2.Hughes, C., Ma, B., Lajoie, G.A.De novoSequencing Methods in Proteomics. Methods Mol Biol. 2010;604:105-21. 3.Ma, B. & Johnson, R.De novoSequencing and Homology Searching. Molecular & Cellular Proteomics. 2012;11(2). (点击图片即可查看活动详情) 如果您想深入了解更多de novo Sequenc...
De Novo Sequencing Methods in Proteomics The review describes methods of de novo sequencing of peptides by mass spectrometry. De novo methods utilize computational approaches to deduce the sequenc... C Hughes,B Ma,GA Lajoie - 《Methods Mol Biol》 被引量: 92发表: 2010年 Protein interaction mapp...
Finally, limitations of current methods are highlighted and new directions are discussed for a wider acceptance of de novo sequencing in the community.Muth ThiloHartkopf FelixVaudel MarcRenard Bernhard Y.Bioinformatics Unit (MF 1)Robert Koch Institute13353 Berlin GermanyProteomics...
De novo peptide sequencing is one of the most challenging topics in the field of computational proteomics. In this manuscript, a novel method based on virtual database searching is presented to improve the performance of de novo sequencing for the data from high resolution LTQ-FT mass spectrometry...
Areas covered: De novo protein sequencing, shotgun proteomics and other mass-spectrometric techniques, along with the various software are currently available for proteogenomic analysis. Emphasis is placed on the methods for de novo sequencing, together with potential and shortcomings using databases for...
PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification Lajoie, B. Ma, PEAKS DB: de novo sequencing assisted database search for sensitive and accurate peptide identification, Mol. Cell. Proteomics 11 (4) (... Z Jing,X Lei,B Shan,... - 《...
一、概述 由于难以获得100%的蛋白氨基酸序列覆盖率,蛋白组de novo测序成为了蛋白测序的难点,由Ac-LysargiNase(N端蛋白酶)和胰蛋白酶构成的镜像酶组合可以解决这个问题并具有稳定性,这2种消化位点互补的酶能够产生目标蛋白的镜像b,y离子,基于镜像原理设计的算法pNovoM
In such cases, the amino acid sequence of the protein has to be determined directly by proteomic techniques. To date, there are only a few reported methods attempting to perform end-to-end de novo protein sequencing by LC-MS/MS, of which ALPS and meta-SPS (mSPS) are among the most ...
To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to build a transcriptome dataset for functional genomics analysis by large-scale RNA sequencing (RNA-seq). Methods To provide a more comprehensive and ...
De novo sequencing software has been widely used in proteomics to sequence new peptides from tandem mass spectrometry data. This study presents a new software tool, Novor, to greatly improve both the... 关键词: Peptide de novo sequencing Tandem mass spectrometry Software Real time Decision tree ...