Tumor cell apoptosis, DNA damage, DNA damage response (DDR) and tumor cell self-renewal were assessed. Protein and mRNA expressions by western-blot and RT-PCR, apoptosis by FACS, cell death by COMET assay and self-renewal by clonogenic assay.Results: Here, we report that increased expression...
DCLK1+ Tuft cells were reported to be required for resti- tution of mouse intestinal crypts in response to inflammation/radiation damage25. Thus the literature so far strongly implicates a possible important role of DCLK1 in mouse colon tumorogenesis and in maintaining the growth of human colon...
Infiltrated neutrophils can worsen the inflammatory response by releasing some mediators, which causes damage to the respiratory tract tissue, mucus hypersecretion, and insensitivity to steroids [12, 13, 14]. This persistent con- dition can eventually result in fibrosis of the respiratory tract and ...
We found that small intestinal crypts of Villin;Dclk1mice were hypoplastic and more apoptotic 24鈥塰 post-total body irradiation, a time when stem cell survival is p53-independent. Injury-induced ATM mediated DNA damage response, pro-survival genes, stem cell markers, and self-renewal ability ...
Mo1805 Ablation of DCLK1 in Intestinal Epithelium Exacerbates Colonic Epithelial Barrier Damage in Response to DSS TreatmentDeveloping novel strategies to isolate and validate stem cells in DNA damage response and injury repair and regeneration.
Cell self-renewal, secondary and tertiary organoid generation, survival, DNA damage, DNA damage response (DDR) and apoptosis were assessed. Protein and mRNA expressions by western-blot and RT-PCR.Results: Here, we report that increased expression of Dclk1 is the key to the generation of tumor...
DCLK1+ Tuft cells were reported to be required for restitution of mouse intestinal crypts in response to inflammation/radiation damage25. Thus the literature so far strongly implicates a possible important role of DCLK1 in mouse colon tumorogenesis and in maintaining the growth of human colon ...