Utilizing DAPI-stained cells in fluorescence microscopy enables researchers to visualize the nucleus. Common protocols call for either live or fixed cells. Fixed cells are typically incubated in DAPI for 5-10 minutes, then rinsed in PBS or TBS-T multiple times to remove excess stain. Once ...
At 48 hours cells were fixed and stained with anti-Tau (MN1000), followed by Goat anti-Mouse Alexa Fluor Plus 488 (A32723) and NucBlue??? Fixed Cell ReadyProbes??? Reagent (R37606). Cells were imaged on an M7000 (AMF7000) using an Olympus 20X objective (AMEP4734) with DAPI (AMEP...
1.For adherent cells ( remove the medium in the orifice plate ) or tissue sections, add a small amount of DAPI staining solution and cover the sample. For suspension cells, at least 3 times the volume of the sample to be stained was added and mixed. 2.Cells were cultured at room ...
Now you can start Fiji (usually fiji-win32.exe) and open the DAPI-picture where youwant to count cells using the `File'-menu or just dragging your picture to the programwindow.Ian LevenfusLevenfus, I., 2011, An efficient method for counting DAPI-stained cells using Fiji, IL-PubServ ...
Cells stained with DAPI and imaged with the Thermo Scientific CellInsight High-Content System. Ordering information 货号产品名称规格单价(CNY)数量 D1306 DAPI (4',6-diamidino-2-phenylindole, dihydrochloride), 10 mg, 10 mg Each 1,493.00 飞享价 1,990.00 截止至: 2024/12/31 订购...
7、channelmeanofG2/Gl,G3/G1,andG4/G1wereabout2,3,and4respectively.BothCVvaluesofthefirstpeakwere2.4.Therewere2peaksonCTNhistogramsandtheratioofpeakchannelmeanofG2/G1was1.97.andCVvalueofGO/G12.4.ThecompletecellcycleofHT29cellsstainedwithDAPI.Hoechst33342orP1wasshowedentirely,Cvalueswere3.40,3.02...
Objective:To discuss a simple method for flow cytometric analysis of cell DNA stained with DAPI and Hoechst33342.Methods:HT 29 cells stained with DAPI,Hoec... XJ Liu,HR Ding,H Zhang - 《Journal of Peking University》 被引量: 13发表: 2010年 Hoechst33342与DAPI标记细胞核对胞内活性氧检测效果...
Anticancer activity of liposomal bergamot essential oil (BEO) on human neuroblastoma cells Citrus extracts, particularly bergamot essential oil (BEO) and its fractions, have been found to exhibit anticancer efficacy. However, the poor water solub... C Celia,E Trapasso,M Locatelli,... - 《...
1. DAPI dye is more sensitive to staining mammalian cells than staining bacteria. It is recommended to stain with a final concentration of 10 μg / mL in PBS or 150 mM NaCl for 30 min at room temperature to stain bacteria. Dead cells are usually stained more brightly than live cells. ...
Cells stained with DAPI and imaged with the Thermo Scientific CellInsight High-Content System. Ordering information 货号产品名称规格单价(CNY)数量 D1306 DAPI (4',6-diamidino-2-phenylindole, dihydrochloride), 10 mg, 10 mg Each 1,493.00 飞享价 1,990.00 截止至: 2024/12/31 订购...